简介：Previousstudieshavedemonstratedthatdoublecortin-positiveimmatureneuronsexistpredominantlyinthesuperficiallayerofthecerebralcortexofadultmammalssuchasguineapigs,andtheseneuronsexhibitveryweakpropertiesofself-proliferationduringadulthoodunderphysiologicalconditions.Toverifywhetherenvironmentalenrichmenthasanimpactontheproliferationandmaturationoftheseimmatureneuronsintheprefrontalcortexofadultguineapigs,healthyadultguineapigsweresubjectedtoshort-termenvironmentalenrichment.Animalswereallowedtoplaywithvariouscognitiveandphysicalstimulatingobjectsoveraperiodof2weeks,twiceperday,for60minuteseach.Immunofluorescencestainingresultsindicatedthatthenumberofdoublecortin-positivecellsinlayerIIoftheprefrontalcortexwassignificantlyincreasedaftershort-termenvironmentalenrichmentexposure.Inaddition,thesedoublecortin-positivecellsco-expressed5-bromo-2-deoxyuridine(amarkerofcellproliferation),c-Fos(amarkerofcellviability)andNeuN(amarkerofmatureneurons).Experimentalfindingsshowedthatshort-termenvironmentalenrichmentcaninduceproliferation,activationandmaturationofdoublecortin-positivecellsinlayerIIoftheprefrontalcortexofadultguineapigs.

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