简介:The’polarcoding’proposedbyDr.Arkancanachievethesymmetriccapacityofbinary-inputdiscretememorylesschannels(B-DMC).ThegeneratormatrixofpolarcodesisGN=BNFnforN=2n,BNwasapermutationmatrix.Inthearticleitwasrealizedwithaninterleaver,sothematrixproductionofGNwasavoided;thenthegeneratormatrixwasjustdeterminedbythematrixFnwhichwasconstructedwiththreesub-matrixesofFn-1andone2N-1orderzeromatrix,itwasdealwithfastHadamardtransform(FHT)algorithm.Thecomplexityofthenewschemewasreducedsharply,andaniterativealgorithmalsocanbeused.TheexampleshowedthatwhenN=8,complexityoftheencodingschemewasjust16whichisobviouslylessthanthatoforiginalencodingscheme36.
简介:Thispaperpresentssomeresultsoftherelationbetweenwavelettransformandfractaltransform.Thewavelettransformoftheattractoroffractaltransformpossesestranslationalandscaleinvariance.Sowespeedthefractalimageencodingbytestingtheinvarianceofthewavelettransformappropriateforimageencoding.Theclassficationschemeofrangeblocksbywavelettransformisgiveninthispaper.
简介:Inthispaper,anewpseudocolormethodtodisplaysoundholospectrogramisproposedandstudied.Onthepictureofsuchafour-dimensionalspectrogram,thehorizontalaxisreferstotime,theverticalaxistofrequency,thecolortophase,andthebrightnesstomagnitude.Anexampleofdisplayisgivenbyemployingtherealizedsystem.
简介:Manymonographspointoutthatdifferentialencodinganddecodingisnecessaryforef-fectualinformationtransmissionagainstphaseambiguitywhileseldomdiscussthereasonwhyphaseambiguitywillemergeinevitably.Availablealgorithmsarespeciallydesignedforcertainmodulationscheme;thesealgorithmscannotsatisfytherequirementofsoft-definedradio,whichperhapsdemandsauniformalgorithmfordifferentmodulations.Thispaperproposesanewopiniononphaseambiguityfromtheviewofprobability.Thisopinionbelievesthatmodulatingsymbolsequencecanaffect,atoptimumsamplingepoch,themodulatedwaveformasoscillatingcarrierhasdone,andsothestochasticsequenceleadstophaseambiguity.Basedonageneralsignalmodel,thispaperalsoputsforwardanoveluniversalalgorithm,whichissuitablefordifferentsignals,evensomenewones,byconfiguringseveralparameters.
简介:Oceanunderwaterexplorationisapartofoceanographythatinvestigatesthephysicalandbiologicalconditionsforscientificandcommercialpurposes.Andvideotechnologyplaysanimportantroleandisextensivelyappliedforunderwaterenvironmentobservation.Differentfromtheconventionalmethods,videotechnologyexplorestheunderwaterecosystemcontinuouslyandnon-invasively.However,duetothescatteringandattenuationoflighttransportinthewater,complexnoisedistributionandlowlightconditioncausechallengesforunderwatervideoapplicationsincludingobjectdetectionandrecognition.Inthispaper,weproposeanewdeepencoding-decodingconvolutionalarchitectureforunderwaterobjectrecognition.Itusesthedeepencoding-decodingnetworkforextractingthediscriminativefeaturesfromthenoisylow-lightunderwaterimages.Tocreatethedeconvolutionallayersforclassification,weapplythedeconvolutionkernelwithamatchedfeaturemap,insteadoffullconnection,tosolvetheproblemofdimensiondisasterandlowaccuracy.Moreover,weintroducedataaugmentationandtransferlearningtechnologiestosolvetheproblemofdatastarvation.Forexperiments,weinvestigatedthepublicdatasetswithourproposedmethodandthestate-of-the-artmethods.Theresultsshowthatourworkachievessignificantaccuracy.Thisworkprovidesnewunderwatertechnologiesappliedforoceanexploration.
简介:TostudytheexpressioncharacteristicofJapaneseencephalitisvirus(JEV)prMEandEproteinsandtheefficacyofDNAimmunizationbydifferentrecombinantplasmidscontainingJEVprME(2001bp)andE(1500bp)genes,tworecombinants(pJMEandpJE)containingJEVprMEandEgenesfusedwithFLAGwereconstructedandthentransfectedintoHepG2andCOS-1cellsbylipnsomefusion.TheexpressionfeatureofFLAG-prME(about72kDa)andFLAG-E(about54kDa)proteinsintransfectedcellswereanalyzedbyWesternblotandtwoantibodysystems(anti-FLAGandanti-E).BALB/cmicewereimmunizedwith100μgoftwokindsofrecombinantsbyintramuscularinjection,andJEVJaGAr-01strains(10^5PFU/100μl)weregiventoBALB/cmicebyintraperionealinjection3wkaftertwiceDNAimmunizationbyalethalviruschallenge.BALB/cmicewereobservedfor21daysafterchallenge.80%plaquereductionneutralizationtestwasperformedtotitrateneutralizationantibodybeforeandafterviralchallenge.ItwasfoundthattheexpressionofproteinsassociatedwithpJMEandpJEwasdeterminedintransfectedcellswithanti-FLAGandanewproteinof11kDawasdetectedinHepG2andCOS-1cellstransfectedwithpJME.OnlyE(53kDa)proteinwasidentifiedastransfectedwithpJMEusingantiE.HigherlevelofneutralizationantibodiesandtheefficacyofprotectiveimmunitywereinducedwithpJMEimmunization,andweresimilartothoseinducedbyinactivatedJapaneseencephalitisvaccine,butwerebetterthanthoseinducedwithpJE.ItconcludesthattheexpressionlevelfromprMtoEproteinsofJEVisdifferentinvitro,andtheinvitroexpressionefficiencyofpJMEwasbetterthanthatofpiE.FLAG-prMEproteinexpressedbypJMEcouldbecleavedbypeptidasefromhost.TheefficacyofDNAimmunizationiscorrelatedtotheexpressioncharacterizationofrelatedproteinsexpressedinvitro.
简介:Polyamines在在植物,而是他们的准确角色调整各种各样的发展过程被含有并且他们怎么管理这些过程尚待逃犯。我们这里报导浓密的Arabidopsis和矮子异种,bud2,哪个从编码S-adenosylmethioninedecarboxylases(SAMDC)的小基因家庭的一个成员的完全的删除的结果为在polyamine简历的不可缺少的中介的形成必要合成小径。bud2植物在开花期,根,和叶柄扩大了脉管的系统,并且polyamines的改变的动态平衡。bud2和samdc1的双异种,另一个SAMDC成员的击倒的异种,是胚胎致命,证明SAMDC为植物胚胎开始是必要的。我们的结果建议polyamines为更高的植物的正常生长和发展被要求。
简介:鉴于伪周期的低密度的同等值检查(QC-LDPC)的编码复杂性编码的问题,高,最小的距离不足够大它导致错误修正表演的降级,基于完成式,周期的差别设置的新不规则的type-IIQC-LDPC代码(CDS)被构造。这些type-IIQC-LDPC代码的同等值检查矩阵与0的重量由零个矩阵组成,有1的重量的circulant排列矩阵(CPM)和有2的重量(W2CMs)的circulant矩阵。在同等值检查矩阵的W2CMs的介绍使完成能改进错误的更大的最小的距离可能--代码的修正性能。这些代码的更黝黑的图没有girth-4,因此,他们有优秀译码集中特征。另外,因为同等值检查矩阵有伪双的斜结构,快编码算法能有效地减少编码复杂性。新type-IIQC-LDPC编码的模拟结果表演能完成更优秀的错误修正表演并且没在添加剂白人Gaussian噪音(AWGN)上有错误地板现象有和产品算法(矿泉)的隧道反复的译码。
简介:瞄准:由一个树枝状的房间(DC)调查反肿瘤免疫鼠科的前列腺癌症上的疫苗的编码第二等的淋巴的chemokine基因和肿瘤lysate。方法:从C57BL/6的骨头髓的DC是有表示第二等的淋巴的chemokine(SLC)的原生质标志向量的transfected由Lipofectamine2000的cDNA脂肪的一些和肿瘤lysate。从SLC+lysateDC提取的全部的RNA被用来由反向的transcriptase聚合酶链反应(RT-PCR)验证SLC的表示。免疫在鼠科的前列腺癌症上疫苗的DC的治疗学的效果被估计。结果:当与SLC相比DC,lysateDC,DC或磷酸盐缓冲答案(PBS)时,我们发现在C57BL/6鼠标的前列腺肿瘤模型,SLC+lysateDC的adminstration最显著地禁止了肿瘤生长对应物(P<0.01)。荧光灯的染色分析显示出的Immunohistochemical在确定的肿瘤以内的更多的CD4+,CD8+T房间和CD11c+DC的渗入由比另外的DC疫苗疫苗的SLC+lysateDC对待(P<0.01)。结论:编码第二等的淋巴的chemokine和肿瘤lysate的DC疫苗能由CD4+,CD8+T房间和DC的渗入得到重要的反肿瘤免疫,它可能为前列腺癌症提供一个潜在的免疫疗法方法。
简介:Directgenetransferintosomatictissueinvivoisadevelopingtechnologywithpotentialapplicationforcancergenetherapy.Retrovirusvector,whichwasaneffectivevehicle,stillhassomedisadvantagesingeneratinghightiterrecombinantvectorsandmanipulatingtomediateinvirogenetransfer.Inthispaper,recombinantvacciniavirusvectorencodinghuman
简介:幼鳕蛾是苹果和世界范围的梨的一个主要害虫。这只昆虫怎么对环境应力作出回应的增加的知识将改进措施对它使用了的地和收获以后的控制。小热吃惊蛋白质(sHsps)在对环境stressors的细胞的回答起一个主要作用。一份退化oligonucleotide教材,对保存-crystallin领域设计了,在互补DNA(cDNA)结束反应的3快速的扩大被使用放大编码在幼鳕蛾房间线表示的sHsps的抄本,Cp169,使遭到了加热吃惊。三全身的cDNAs从包含了编码sHsps的开的读物框架的Cp169房间被克隆。为CpHsp19.8的cDNA是编码177氨基酸的795bp。为CpHsp19.9的cDNA是编码175氨基酸的749bp。为CpHsp22.2的cDNA是编码192氨基酸的737bp。三CpHsps的蛋白质序列的分析与相同显示了83氨基酸的存在到-crystallin领域。为每CpHsps,-crystallin领域被分叉的N终端和C终端区域包围,与sHsps的保存结构的特征一致。实时聚合酶链反应,过去常决定每在幼鳕蛾的不同发展阶段的sHsps的表示模式在测试的所有阶段揭示了抄本的存在。与另外的sHsps的特征一致,当昆虫被使遭到加热吃惊时,CpHsp抄本的表示极大地被提高。这研究的结果能被用作一个指南用各种各样的收获以后的处理在幼鳕蛾控制学习sHsps的角色。
简介:Thelight-harvestingchlorophylla/b-proteincomplexplaysanimportantroleinphotosynthesisofplants.Afull-lengthcDNAoflight-harvestingchlorophylla/b(cab)genewasclonedfromthefirststrandofMoso(Phyllostachysedulis)cDNAthroughRT-PCRandRACEmethods,namedascab-PhE10(cabgene10fromPh.edulis).Thelengthofcab-PhE10(GenBankaccessionnumber:EU118754)is1151bp,whichcontainsanopenreadingframeencoding283aminoacidsfrom81stto932ndposition.Thebioinformaticsanalysisindicatedthattheproteinencodedbycab-PhE10hadachlorophlla/bbindingdomain(83rd-247thposition),twoproteinkinaseC-phosphorylationsites,threeN-myristoylationsitesandayiaA/Bdoublehelixdomain.Theaminoacidsequenceofcab-PhE10showedhighsimilaritywiththecabgenesofOryzasativa,Zeamays,Hordeumvulgare,andVitisvinifera,morethan80%,respectively,whichindicatedthatcab-PhE10genebelongstolhcb5genefamily.
简介:Smallubiquitin-likemodifier(SUMO)-conjugatingenzymesareinvolvedinpost-translationalregulatoryprocessesineukaryotes,includingtheconjugationofSUMOpeptidestoproteinsubstrate(SUMOylation).SUMOylationplaysanimportantroleinimprovingplanttolerancetoabioticstresssuchassalt,drought,heatandcold.Herein,wereportedtheisolationofOsSCE1(LOC_Os10g39120)geneencodingaSUMO-conjugatingenzymefromrice(Oryzasativacv.Nipponbare)anditsfunctionalvalidationinresponsetodroughtstress.TheE2enzyme,OsSCE1,isoneofthreekeyenzymesinvolvedintheconjugationofSUMOtoitstargetproteins.ActivatedSUMOistransferredtothecysteineofanE2enzymeandthentothetargetlysineresidueofthesubstrate,withorwithoutthehelpofanE3SUMOligase.ExpressionofOsSCE1wasstronglyinducedbypolyethyleneglycol6000(PEG6000)treatment,whichsuggestedOsSCE1maybeinvolvedinthedroughtstressresponse.OverexpressionofOsSCE1(OsSCE1-OX)inNipponbarereducedthetolerancetodroughtstress.Conversely,thedroughttolerancewasslightlyimprovedbytheknockdownofOsSCE1(OsSCE1-KD).TheseresultswerefurthersupportedbymeasurementofprolinecontentinOsSCE1-OXandOsSCE1-KDtransgeniclinesunderinduceddroughtstress,whichshowedOsSCE1-KDtransgeniclinesaccumulatedhigherprolinecontentthanthewildtype,whereasOsSCE1-OXlinehadlowerprolinecontentthanthewildtype.ThesefindingssuggestedOsSCE1mayplayaroleasanegativeregulatorinresponsetodroughtstressinrice.
简介:Low-DensityParity-Check(LDPC)codeisoneofthemostexcitingtopicsamongthecodingtheorycommunity.Itisofgreatimportanceinboththeoryandpracticalcommunicationsovernoisychannels.ThemostadvantageofLDPCcodesistheirrelativelylowerdecodingcomplexitycomparedwithturbocodes,whilethedisadvantageisitshigherencodingcomplexity.Inthispaper,anewap-proachisfirstproposedtoconstructhighperformanceirregularsystematicLDPCcodesbasedonsparsegeneratormatrix,whichcansignificantlyreducetheencodingcomplexityunderthesamede-codingcomplexityasthatofregularorirregularLDPCcodesdefinedbytraditionalsparseparity-checkmatrix.Then,theproposedgenerator-basedsystematicirregularLDPCcodesareadoptedascon-stituentblockcodesinrowsandcolumnstodesignanewkindofproductcodesfamily,whichalsocanbeinterpretedasirregularLDPCcodescharacterizedbygraphandthusdecodediteratively.Finally,theperformanceofthegenerator-basedLDPCcodesandtheresultantproductcodesisinvestigatedoveranAdditiveWhiteGaussianNoise(AWGN)andalsocomparedwiththeconventionalLDPCcodesunderthesameconditionsofdecodingcomplexityandchannelnoise.
简介:早成熟的indica米饭变化Zhefu49,质量和淀粉组织其谷物,对环境温度敏感,在在植物生长房间充满舞台的谷物受到不同温度(为高温度的32°C和为最佳温度的22°C),并且编码淀粉分叉的三isoform基因(SBEI,SBEIII和SBEIV)的不同表情在内乳的酶(SBE)被即时荧光学习量的PCR(FQ-PCR)方法。在开发米饭内乳的SBE表示上的高温度的效果是isoform依赖的。高温度显著地下面调整SBEI和SBEIII的表情,当时起来调整SBEIV的表示。与SBEIV和SBEIII相比,在Zhefu49米饭内乳的SBEI基因的表示对在充满舞台的谷物的温度变化更敏感。这研究显示那特别处于天气/气候条件变化由isoform表示证实了的温度压力影响米饭谷物形成和它的质量。
简介:Oncolysate,adebrisoftumorcells,hasbeenproventobeeffectiveintumoractiveimmunotherapy,itwasreportedthatthevacciniavirus,especiallyrecombinantvacciniavirusesencodinghumanIL-2(rVV-IL-2),enhancedtheimmunogenicityoftransfectedtumorcells.Inthisexperiment,themurinemelanomacellB16-F10oncolysatestransfectedbyrVV-IL-2(IL-2VBO)wereusedasvaccine.TheIL-2VBOorTK-VBOwaspreparedbyincubatingB16-F10cellswithrVV-IL-2orrVV-TKat