简介：ThekinematicsofturningmaneuversofstartledCrucianCarp(Carassiusauratus)arepresented.AllescaperesponsesobservedareC-typefast-starts.Thepositionofthecenterofmassandthemomentofinertiaofthefisharecalculated.Theresultsshowthatthepositionofthecenterofmassisalwaysat35%ofthelengthofthefishfromtheheadandthepositionofthecenterofmassandmomentofinertiacanbeconsideredunchangedduringC-startofCrucianCarp.HydrodynamicanalysisoftheC-startisgivenbasedonthekinematicsdatafromourexperiments.TheC-startconsistsofthreestages.Instage1,thetailfinoffishrapidlyflapsinonedirection,andalargemomentactsonthefish'sbody,whichrotatesaroundthecenterofmasswithanangularacceleration.Instage2,thetailfinflapsmoreslowlyintheoppositedirectionatslowerspeed,thefish'sbodyrotatesaroundthecenterofmasswithangulardecelerationandthecenterofmassofthefishmovesalonganarc.Instage3,themomentapproximatelyequalszero,thefish'sbodystopsrotatingandthecenterofmassthemovesalongastraightline.

简介：目的:利用Adeasy-1系统,构建并鉴定CARP基因重组腺病毒载体.方法:PCR扩增含有CARP全长cDNA的片段,经测序验证无误后,亚克隆至pAdTrack-CMV穿梭质粒,再与pAdEasy-1质粒在大肠杆菌BJ5183中进行同源重组产生腺病毒载体质粒.经过抗性筛选以及酶切鉴定得到阳性的重组质粒,再在293细胞中进行包装扩增,利用Adeasy系统上的绿色荧光蛋白标签鉴定病毒表达.结果:测序证实PCR产物为CARP全长cDNA;抗性筛选及酶切鉴定均表明重组腺病毒载体构建成功;转染293细胞3天后可见绿色荧光,回收病毒可以重复感染293细胞,证明病毒包装成功.

简介：Thestudyiscarriedoutontheeffectofdrillingnoiseandvibrationongrowthofgrasscarp,MyloparyngodonPiceus,byusingcut-finmarkingmethodinsitu.Comparedwithothermethods,themethodismoreappropriate,foritsoperationissimplerandmoredatamaybeobtainedunderthesamecondition.Theresultsshowthatdrillingnoiseandvibrationhavesignificanteffectonthegrowthofgrasscarp.Criticalequivalentnoiseandvibrationgrade(NleqandVleq)areabout84.4dBand90.2dB,andtheaffectingradiusisabout8.5m.Theeffectofdrillingnoiseandvibrationcouldbeinfluencedbysomefactors,suchasdurationofpollutionandbodyweightofgrasscarp,etc.Grasscarp′sgrowthcouldrapidlyrecoverafterremovingdrillingnoiseandvibration,indicatingthatthedrillingnoiseandvibrationdonotdamagethefishorgansandtheeffectisreversible.Therefore,theeffectmechanismmaybeduetotheactivatingresponseofnon-hearingsystem.

简介：表示顺序标注的普通鲤鱼(EST)为存在ofmicrosatellites，或简单顺序重复(SSR)被分析。在NCBIdbEST数据库，10612sequences的一个总数在2004年12月31日前被登记。2-6nucleotidemicrosatellites的完全的搜索导致了513包含SSREST的鉴定，为4.8%总数的财务。聚合分析显示包含SSREST的73个序列掉进27个组并且留下440EST是独立的。467唯一的包含SSREST的一个总数被识别。这些EST-SSRs包含了许多简单顺序类型，并且di-nucleotiderepeats和tri-nucleotiderepeats是最丰富的，为42.1%全部和27.9%的财务分别地。thedinucleotide重复，CA/TG是最丰富的，由GA/TC列在后面。BLASTx搜索证明SSRloci的that38.1%能与基因或蛋白质被联系知道或包含SSREST的未知function.BLASTx搜索也在zebrafishsequences上显示出点击的高频率(98/179)。