简介:【摘要】目的: 调查 皮肤性病门诊患者泌尿生殖器Ⅱ型单纯疱疹病毒( HSV-Ⅱ) 、解脲脲原体( UU) 、沙眼衣原体( CT) 、淋病奈瑟菌( NG) 感染情况,分析干预方式。 方法: 选 85 例疑似泌尿生殖器 Ⅱ型 HSV-Ⅱ感染患者,同时选 81 例疑似 UU、 CT、 NG感染患者,采用荧光定量聚合酶链反应法,所有患者均行脱氧核糖核酸检验,比较检验结果。 结果: 不同性别 HSV-Ⅱ阳性检出率比较,差异不显著( Χ2=3.057 , P=0.080 ); 不同年龄分组,其阳性检出率比较,差异不显著( P > 0.05 )。 UU阳性检出率比较,女性较高( P < 0.05 ), CT、 NG不同性别阳性检出率比较,差异不显著( P > 0.05 ),不同年龄段比较, 20 ≤年龄≤ 40 组高于 ≤ 19 岁组( Χ2=7.884 , P=0.005 ),且高于 41 ≤年龄≤ 60 组( Χ2=4.309 , P=0.037 )。 结论: 皮肤性病门诊患者泌尿生殖器Ⅱ型 HSV-Ⅱ、 UU、 CT、 NG感染发生率较高, HSV-Ⅱ感染不同性别、不同年龄差异均不显著, UU、 CT、 NG感染存在年龄与性别差异。
简介:摘要目的探讨Ⅱ型单纯疱疹病毒(HSV)抗体检测在生殖器疱疹(GH)诊断中的应用价值。方法本文选取确诊典型生殖器疱疹患者及不典型生殖器疱疹患者得血清标本检测Ⅱ型单纯疱疹病毒抗原、抗体,并评价HSV-Ⅱ抗体检测在生殖器疱疹诊断中的应用价值。结果HSV-Ⅱ抗原检测阳性率为56.52%(39/69),HSV-Ⅱ抗体检测阳性率为94.20%(65/69),经统计学分析发现HSV-Ⅱ抗体检测阳性率明显高于HSV-Ⅱ抗原检测阳性率,且差异具有统计学意义(χ2=8.26,P<0.05)。结论对临床表现典型的GH患者检测HSV-Ⅱ抗原,无症状或临床表现不典型的可疑病例检测HSV-Ⅱ抗体,可有效提高GH的诊断率。
简介:摘要目的探讨HPV、HSV-Ⅱ病毒基因检测配合电子阴道镜检查在宫颈癌前病变早期诊断及治疗后随访的临床意义。方法对286例初诊可疑宫颈病变患者,及82例经病理确诊为CIN的患者行跟踪性HPV、HSV-ⅡDNA检测,TCT检查及电子阴道镜检查。结果286例初诊的可疑宫颈病变患者宫颈癌、CIN及宫颈炎的HPV阳性率分别为92.30%、59.26%、14.58%;HSV-Ⅱ阳性率分别为61.54%、30.86%、8.85%,宫颈病变严重程度与HPV、HSV-Ⅱ感染率呈正比关系。82例确诊为CIN随访的患者,经阴道镜检查及活检发现CIN再复发6例,复发率7.32%,6例HPV均阳性,相同基因型4例,不同基因型2例;HSV-Ⅱ阳性3例,HSV-Ⅱ阴性3例。CIN再复发与病毒未清除及新的感染相关。结论HPV-DNA、HSV-ⅡDNA检测、TCT检查配合阴道镜检查可显著提高宫颈癌前病变的诊断水平,可早期发现CIN患者治疗后再复发。
简介:摘要目的比较单纯性疱疹病毒(HSV)抗原和HSV-2型IgM抗体检测法诊断生殖性疱疹感染的临床效果。方法回顾性分析我站收治的188例生殖性疱疹疑似病例经直接免疫荧光法(DFA)检测疱液样本中的HSV抗原和ELISA法检测血清中的HSV-2IgM的检测结果,以免疫印迹法(WB)为标准,比较HSV-2IgM抗体检测法和DFA敏感度及特异性。结果188例患者确诊106例为阳性,其中原发感染30例,复发感染76例;在原发感染诊断中HSV-2IgM抗体检测法与DFA敏感度和特异性比较,差异无统计学意义(P>0.05);在复发感染诊断中HSV-2IgM抗体检测法与DFA敏感度比较,差异具有统计学意义(P<0.01);HSV-2IgM抗体检测法与DFA总敏感度比较,差异具有统计学意义(P<0.01)。结论DFA反应迅速、操作简单,在生殖性疱疹感染诊断中敏感度和特异性均较高;HSV-2IgM抗体检测法对于原发感染诊断敏感度和特异性较高,可将两种方法结合诊断效果更佳。
简介:Inordertoacknowledgethemulti-infectiondataofSTDpatientsandimprovetheprophylaxisandtherapyforSTDs,297patientsand30healthypeoplewereexaminedusingtheHSV-2plasmaantibody.TheresultsshowedthatallkindsofSTDpatientswereinfectedbyHSV-2indifierentratios.thehighestratiooccurringinsyphilispatients.
简介:摘要目的本研究从血清学及临床保护角度探讨单纯疱疹病毒1型(herpes simplex virus type 1,HSV-1)和HSV-2免疫交叉反应的特征,以期为控制及预防这两种病毒引起的疾病提供数据资料。方法HSV-1减毒株M3免疫小鼠,检测其诱导产生以中和抗体为指征的特异性免疫应答情况。免疫28 d后分别用HSV-1野毒株和HSV-2野毒株经不同途径对小鼠进行攻击感染,观察M3免疫小鼠抵御HSV-1/2病毒感染的情况。结果HSV-1减毒株M3免疫小鼠不能诱导产生特异性的抗HSV-2中和抗体,免疫28 d后接受不同途径的HSV-2攻击,病毒载量显著增加,但未出现明显的异常临床表现,同时组织病理损伤也仅表现为较轻微的炎性反应。HSV-1减毒株M3免疫小鼠能够诱导产生特异性的抗HSV-1中和抗体,并且在HSV-1野毒攻击后表现出明显的保护效果。结论HSV-1减毒株M3免疫小鼠后所诱导的免疫应答对HSV-1野毒株感染攻击表现出以中和抗体为特征的、可限制病毒在体内增殖的免疫保护作用,然而对HSV-2野毒株攻击则表现出不以抗体形式中和病毒而是以控制病毒的增长为主要模式的临床交叉免疫保护能力。
简介:ObjectivesTocomparetheefficiencyoffourcommercialELISAkitsindetectingtype-specificHSV-2IgGantibodies.MaterialsandMethods:Atotalof125subjects,including105withgenitalulcers,and20controlswithoutanyhistoryofSTDswererecruitedfromtheSTDclinicfordetectionoftype-specificHSV-2IgGantibodywithdifferentkits.FourkindsofcommerciallyavailableELISAkits,includingQuidaHSV2IgGELISA(AifulangBiochemCo.Ltd.,Hangzhou),TORCH-HSV2IgG(JingmeiBiotechCo.Ltd.,Shanghai),Captia^TMHSV2IgG(Trinitybiotech,USA)andHerpeSelectT^TM2ELISAlgG(Focustechnologies,USA)wereusedforevaluation.WesternBlotassaywasperformedasagoldstandard.Results:ComparedtoWesternBlotresults,thesensitivityandspecificityofthekits(QuidaHSV2,TORCHHSV2,Captia^TMHSV2andHerpeSelect^TM2)were13.1%and98.4%,7.5%and100%,100%and11.1%,87.7%and96.7%,respectively.Thepositivepredictivevalue(PV)andnegativePVofthefourkitswere88.9%and54.3%,100%and55.5%,55.6%and100%,96.2%and89.2%,respectively.TheareasundertheROCcurveofthreekits(QuidaHSV2,Captia^TMHSV2andHerpeSelect^TM2)were0.885(0.822-0.948),0.825(0.747-0.902),0.974(0.950--0.998),respectivelyConclusion:TheperformanceofHerpeSelect^TM2isthebestamongthefourkits.Theresultsalsoindicatethatthecommerciallyavailablekitsfordetectionoftype-specificHSV2antibodyshouldbere-evaluatedintermsoftheirvalidity.
简介:ToexploretheantiviraleffectandmechanismofpolysaccharidefromSpirulinaplatensis(PSP)onherpessimplexvimstype2(HSV-2),astandardstrainofHSV-2(333strain)wasusedtoinvestigatetheantiviraleffectofPSPinvitro.PSPinvariousconcentrationswasappliedtodifferentstagesofHSV-2replicationcycle.Finally,thevirusinfectivity(TCID50),cytopathiceffect(CPE),andMTTstainingmethodforviablecells(MTTassay)wereusedasmarkerstoevaluatetheeffectofPSPonHSV-2.ThequantityofHSV-DNAwasdetectedbyreal-timefluorescencequantitativePCR(FQ-PCR).TheHSV-2infectedVerocellultrastructureswereobservedbytransmissionelectronmicroscopy(TEM).TheresultsshowedthatPSPhadlittlecytotoxiceffectonVerocells,itcouldnotdirectlyinactivateHSV-2infectivity.PSPnotonlyinterferedinadsorptionofHSV-2toVerocellsbutalsoinllibitedHSV-2biosynthesisinthecells.FQ-PCRresultsshowedthattheinhibitoryrateonHSV-DNAalsoincreasedinadose-dependentandtime-dependentmanner.TEMalsoconfirmedthatPSPexhibitedpronouncedinhibitoryeffectonHSV-2.Inconclusion,theantiviraleffectofPSPonHSV-2maybeattributedtotheinhibitionofvimsadsorption,vimsreplicationandsynthesisincells.
简介:Inviewofthecurrentgesturerecognitionalgorithmbasedonskincolorsegmentationisnotflexibleandhasweakresistancetotheenvironment,thispaperputsforwardanewmethodofskincolormodelingtoimprovetheadaptabilityofgesturesegmentationwhenitfacetodifferentstates.ThemodelingbuiltbydoublecolorspaceinsteadofonlyoneiscompatiblebothinYCbCrandHSVcolorspacetotrainingtheGaussianmodelwhichcanupdatethethresholdvalueforbinarization.Finally,thispaperdesignedanaturalgesturerecognitionandinteractivesystemsbasedonthedoublecolorspacemodel.Ithasshownthatthesystemhasagoodinteractiveexperienceindifferentenvironments.
简介:AbstractIntroduction:Genital herpes is one of the most common sexually transmitted infections worldwide. Most genital herpes occur on the genitalia, perineum, buttocks, upper thighs, or perianal areas. However, there were reports of rare cases of genital herpes on urethral orifice.Herein, we report a case of a patient who was diagnosed with urethral orifice genital herpes, a place likely to be ignored.Case presentation:A 53-year-old woman presented with vagina pruritus at first, with subsequent burning sensation around urethra. Mycostatin and mycospor were administered for the treatment of the pruritus. Then levofloxacin was prescribed. Finally, the patient was given treatment with acyclovir. Different from other cases, the investigation showed herpes simplex virus type 1 DNA positive and herpes simplex virus type 2 DNA negative by PCR. The patient was therefore diagnosed with genital herpes.Discussion:Most genital herpes happen on the genitalia, perineum, buttocks, upper thighs, or perianal areas. Few cases have been reported that genital herpes occurred on urethral orifice till now.Conclusion:If there is no typical symptoms that patient present with herpes simplex virus type 1 or 2 positive, urethral orifice genital herpes should be considered.
简介:摘要目的了解川南地区女性非淋菌性尿道炎(NGU)患者单纯疱疹病毒(HSV)感染情况。方法采用荧光定量聚合酶链反应(FQ-PCR)技术对134例临床诊断为NGU的女性患者和62例参加健康体检妇女生殖道分泌物进行HSV检测。结果134例女性NGU患者中,检测出HSV阳性者36例,阳性率26.87%(36/134);62例健康妇女体检中,HSV阳性者3例,阳性率4.84%(3/62),患者组与对照组比较差异有显著意义(x2=107.45,P<0.05)。结论NGU女性患者容易合并HSV感染,FQ—PCR快速、简便,特异性高,灵敏度强,操作简便,此法用于检测HSV,可诊断且可分型,有利于UGN患者合并HSV感染的早期诊断和及时治疗。
简介:摘要目的分析本地区361例疑似生殖器疱疹(GH)患者单纯疱疹病毒Ⅱ型(HSV-2)脱氧核糖核酸(DNA)检测情况,比较不同性别、年龄和不同季节阳性检出率的差异,为防治HSV-2感染提供参考。方法对2017年8月-2018年1月在我院门诊就诊的疑似GH患者,采取实时荧光定量聚合酶链反应检测HSV-2DNA,对检测结果进行统计分析。结果患者男性占72.30%,女性占27.70%,平均年龄(39.38±13.38)岁,总阳性检出率36.29%,其中男性组阳性检出率34.87%,女性组阳性检出率40.00%,两者差异无统计学意义(P>0.05);不同年龄段阳性检出率不同,但差异无统计学意义(P>0.05);秋季组阳性检出率34.16%,冬季组阳性检出率38.99%,两者差异无统计学意义(P>0.05)。结论本地区HSV-2感染集中于性活跃人群,男女感染比例相当,不同年龄和秋冬两季阳性检出率差异不显著。因此,对所有高危人群及感染性伴侣要随时进行HSV-2的筛查和防治。
简介:建立一种检测HSV2的实时定量PCR,而实时定量PCR检测出HSV2DNA阳性标本有l9例,HSV2的FQPCR定量检测 
简介:Objectiwe:InordertodetecttheroleofmonocytesinHSV-2infection,westudiedtheeffectofherpessim-plexVirus-2infectionontheproductionoftumorne-crosisfactor(TNF-α),interleukin-6(IL-6)secretedbymonocytes.Methods:MonocyteswereinfectedbyHSV-2(333Strain).Culturesupernatantswerecollectedat1,3,5,7dayspost-infection.ThelevelsofTNF-α,IL-6weremeasuredbyenzyme-linkedimmunosorbentas-say(ELISA).Results:ThelevelsofTNF-αsecretionbymono-cytessignificantlydecreasedonfirstdaypost-infection.ThelevelsofIL-6significantlydecreasedonfirstandthirddayspost-infection,andthengradu-allyincreasedtothecontrolonseventhdaypost-infection.Conclusions:TNF-αandIL-6productionbymono-cyteswasinhibitedduringHSV-2infection.Thepro-ductionofcytokinesmayplayanimportantroleinherpessimplexviurs-2pathogenicityandimmunity.