简介：Lasermultipleprocessing,i.e.lasersurfacetexturingandthenLaserShockProcessing(LSP),isanewsurfaceprocessingtechnologyforthepreparationofbionicnon-smoothsurfaces.Basedonengineeringbionics,samplesofbionicnon-smoothsurfacesofstainlesssteel0Crl8Ni9weremanufacturedintheformofreseaustructurebylasermultipleprocessing.Themechanicalproperties(includingmicrohardness,residualstress,surfaceroughness)andmicrostructureofthesamplestreatedbylasermultipleprocessingwerecomparedwiththoseofthesampleswithoutLSPTheresultsshowthatthemechanicalpropertiesofthesesamplesbylasermultipleprocessingwereclearlyimprovedincomparisonwiththoseofthesampleswithoutLSPThemechanismsunderlyingtheimprovedsurfacemicrohardnessandsurfaceresidualstresswereanalyzed,andtherelationsbetweenhardness,comnressiveresidualstressandroughnesswerealsopresented.

简介：Transgeniccelllinesofloblollypine(PinustaedaL.)wereanalyzedbyacompactlaser-tweezers-Raman-spectroscopy(LTRS)systeminthisinvestigation.Alowpowerdiodelaserat785nmwasusedforbothlaseropticaltrappingofsingletransgeniccellsandexcitationfornear-infraredRamanspectroscopyofthenucleiofsynchronizedcells,whichweretreatedassingleorganicparticles,attheS-phaseofthecellcycle.TransgeniclivingcellswithgfpanduidAgeneswereusedasbiologicalsamplestotestthisLTRStechnique.Asexpected,differentRamanspectrawereobservedfromthetestedbiologicalsamples.Thistechniqueprovidesahighsensitivityandenablesreal-timespectroscopicmeasurementsoftransgeniccelllines.Itcouldbeavaluabletoolforthestudyofthefundamentalcellandmolecularbiologicalprocessbytrappingsinglenucleusandbyprovidingawealthofmolecularinformationaboutthenucleiofcells.

简介：Themethodoflasercapturemicrodissection(LCM)combinedwithsuppressivesubtractivehybridization(SSH)wasdevelopedtoisolatespecificgermcellsfromhumantestissectionsandtoidentifythegenesexpressedduringdifferentiationanddevelopment.Inthepresentstudy,over10,000primaryspermatocytesandroundspermatidcellsweresuccessfullyisolatedbyLCM.UsingthecDNAsfromprimaryspermatocytesandroundspermatids,SSHcDNAslibraryofprimaryspermatocyte-specificwasconstructed.TheaverageinsertsizeofthecDNAisolatedfrom75randomlypickedwhitecloneswas500bp,rangingfrom250bpto1.7kb.Usingthedot-blotmethod,atotalof421cloneswereexamined,resultingintheidentificationof390positiveclonesemittingstrongsignals.PartialsequenceofcDNAspreparedfromeachclonewasdeterminedwithanoverallsuccessrateof84.4%.GenesencodingcytochromecoxidaseⅡandtherescuefactor-humaninweremostfrequentlyexpressedinprimaryspermatocytes,suggestingtheirrolesinvolvedinmeiosis.