简介：Heavyionirradiationattractalargeinterestfortwoapplications:radiotherapyandspaceradiationprotectioninmannedspacemissions.ExposuretoheavyionsradiationresultsinmultipleeffectsthroughDNAdamageinduction.Single-cellgelelectrophoresisorcometassayisknownforitsabilitytodetectDNAdamageatthesinglecelllevelandhasbeenusedforyearstoassessDNAdamage.ItcandetectlowlevelsofDNAstrandbreaksinashorttime,justusingafewsamplecells.DNAdoublestrandbreaks(DSBs)aremeasuredattheneutralcometassaycondition;underthealkalinecometassayconditionbothDNAsinglestrandbreaks(SSBs)andpartDSBsaredetected.Thetwodimensionalcometassayisamodificationofthetwooriginalcometassay,cansimultaneouslydetectDNASSBsandDSBsinthesamehumanspermatozoa.

简介：Amongthemanyspecialfactorsinthespaceenvironment,spaceradiationespeciallyhighenergyheavyionradiationiswidelyregardedasoneofthecrucialfactorsthathazardthehealthandsafetyofastronaut.Itiswellknownthatbiologicaleffectsofradiationaredependentonthelinear-energy-transfer(LET)oftheemergentbeam.HeavyionshavehighLETandcouldinducecomplexandclusteredDNAdamage,whichismoredifficulttoberepairedthanindividuallesionsandisevenirreparable.