简介:合成了N,N′-亚水杨基皮考林酰肼(HL)及其铁配合物[FeL2](C26H20FeN6O4,Mr=536.33).X射线衍射实验结果表明,标题配合物晶体属于正交晶系,空间群为Pbcn,晶体学参数:a=1.4970(1)nm,b=1.51556(9)nm,c=2.0920(2)nm,V=4.7462(6)nm3,Z=8,Dc=1.501Mg·m-3,F(000)=2208,μ(MoKα)=0.682mm-1,R=0.0695,Rw=0.1502.在配合物[FeL2]中,铁(Ⅱ)原子具有扭曲的八面体配位构型,晶体通过分子间氢键作用形成缔合分子对.红外光谱表明,配体在形成配合物后,ν(CO)和ν(CN)红移.电子光谱表明存在π-π*和d-π*的跃迁;荧光光谱表明,配合物金属对配体n-π*激发引起的荧光发射峰有较大的影响.
简介:采用HF/6-31G*方法优化分子构型,在此基础上用CPHF方法系统地研究了多种基团取代的2-苯基苯并咪唑衍生物的二阶非线性光学系数βvec,并对βvec的影响因素进行了探讨,为进一步设计综合性能优良的有机非线性光学材料提供理论指导.
简介:Aself-assembledmonolayerfilm(SAM)ofrutheniumphthalocyanine(RuPc)fabricatedonasilversubstratepremodifiedwithanSAMofterephthalonitrile(TPN)wasstudiedbymeansofsurface-enhancedRamanscattering(SERS)andultraviolet-visible(UV-Vis)spectroscopies.TPNwasusedasaligandtolinkRuPcsinceitcannotonlymodifythesilversubstratebutalsodeliverthenitrilegroupsprotrudingfromthesilversurface.Therefore,wecanexploretherelationshipbetweenthestructureandtheorientationofRuPcandtheTPN-modifiedsubstrate.TheUV-VisspectraindicateastronginteractionbetweenRuPcandTPNinthecompositefilm.TheresultisfurtherconfirmedbytheSERSspectraofRuPc-TPNSAM,inwhichthevibrationalbandsarisingfromboththeRuPcandTPNmoietiesappearclearly,indicatingthattheRuPcissuccessfullyassembledontheTPNfilm.
简介:Toinvestigatethetreatmenteffectof2-seleniumbridgedβ-cyclodextrin(2-SeCD),aGPXmimic,onthestrokeofstroke-pronespontaneouslyhypertensiverats(SHRSP),fifty-twoSHRSPof8-weekoldwererandomlydividedintofourgroupsA,B,CandcontrolgroupD.TheratsofgroupsA,B,CandDweregiven1.0%-1.5%NaClmassfractionasdrinkingfluid.Afteronsetofstroke,groupsA,BandCweregivenorally16.05,160.5and1605mg*kg-1*day-1of2-SeCD,respectively,andgroupDwasgivenwaterfor2weeks.Theclinicalscoreofstroke,systolicbloodpressure(SBP),survivaltimeofratswererecordedandthehistopathologicexaminationsoftheirbrainandcarotidarteryweremadeafterdecapitation.Theclinicalscoresofstrokeaftertreatmentwith160.5mg*kg-1*day-1(GroupB)and1605mg*kg-1*day-1(GroupC)of2-SeCDare2.55±0.98and1.98±0.79,respectively,thoseareobviouslylowerthanthatofgroupD(3.41±0.83,p<0.01).ThesurvivaldaysingroupB(10.0±8.6)andgroupC(14.4±7.9)arelongerthanthatforgroupD(4.7±2.9,p<0.01).TheelectronmicroscopestudyshowedthattheendotheliumofcarotidarterywasneartonormalingroupBandgroupC,whileitwasseriouslyinjuredincontrolgroupDandmildlyinjuredingroupA.2-SeCDmayeffectivelybeusedtotreatthestrokeforSHRSP.
简介:Whenaproteinisencapsulatedintopoly(DL-lactide-co-glycolide)(PLGA)microspheresbymeansofthedouble-emulsionmethod,theharshmicrospheresformationprocessincludingultrasonification,exposuretoanorganicsolventandapolymermaycausethedenaturationoftheprotein.Inthisstudy,weinvestigatedtheenzymaticactivitychangeandtheeffectoftheexcipientsonthestabilityofrecombinanthumanCu,Zn-superoxidedismutase(rhCu,Zn-SOD)duringtheemulsification.ThespecificactivityrecoverywasfoundtobeconcentrationdependentandtheexcipientsinvolvedsuchasPEG600andTween20,andtrehalosewereshowntoincreasethestabilityofrhCu,Zn-SOD.TheproteinstructuralintegritywithinthemicrosphereswasanalyzedbyFTIR.ThestructureofrhCu,Zn-SODwithinPLGAmicrospherescontainingtrehalosewasfoundtobesimilartothatofthenativesolidstate,whereastheproteinencapsulatedduringthepreparationintheabsenceofanyexcipientchangedduetothepossiblehydrophobicinteractionwiththepolymer.Theresultssuggestthatarationalstabilitystrategyforproteintobeencapsulatedintomicrospheresshouldaimatdifferentprocesses.
简介:TofindoptimalconditionsforexpressingthesolubleformofsFv-2F3andtostudythepurificationandpropertyofitsderivativeSe-sFv-2F3,thepreferredexpressionconditionswereinvestigatedbymeansoforthogonaldesign.Thesecultureconditionsincludedincubationtemperature,inducerconcentration,inductiontimeandcellconcentration.TheevaluationofexpressionwasaccomplishedbytheanalysisofwholecelllysatesandtheyieldofsolublesFv-2F3wascalculatedaccordingtotheanalysisofProfinder(FTI-500,Pharmacia).Thepurificationprocedurewascarriedoutviaatwo-steppurificationprocedureconsistingofion-exchangechromatography,followedbyimmobilizedmetalaffinitychromatography(IMAC).TheantioxidantefficacyofSe-sFv-2F3wasdemonstratedbythedeterminationofthecontentofthemainproductoflipidperoxidation,MDA,theviabilityofcellsandtheactivityofLDH.WeobtainedthepreferredcultureconditionstogrowtheengineeredbacteriaandtheprocedureforpreparingsolublesFv-2F3andconfirmedtheantioxidantefficacyofSe-sFv-2F3.
简介:Inanattempttofindnewantitumoragents,anovelclassofchromonecompoundswithabenzimidazoleorabenzoxazoleringinpositions2or6weresynthesizedviacondensationinpolyphosphoricacid(PPA)byusingchromoneacidsasthestartingmaterials.Duringthepreparationprocess,itwasfoundthatPPAcouldcleavethechromoneringtoproducearing-openingcompound(6).Themolarratioofthechromonecompound(5)tothering-openingcompound(6)variedwiththechangeofreactiontemperatureandtime.BasedonMTTprotocol,theantitumoractivityofeachofthecompoundsobtainedwasevaluatedagainstthreehumancancercelllines:KB(oralepidermal),A2780(ovary)andBel7402(liver).TheIC50variedfrom54.7μmol/Ltomorethan180μmol/L.
简介:TakingthetransportoftotalphosphorpollutantsintheBeijingMiyunreservoirforexample,wehaveobtainedthreedimensionaldistributingregularityoftotalphosphorpollutantsbythecalculationofthelinearinterpolationvalueofeachpointbetweenhorizontallayers.Thecredibilityanalysisinallusiontothismethodwascarriedoutandtheprogrammingschemeforrealizingthismethodwassetforth.
简介:Theanodicdischargeofwatertoproduceadsorbedhydroxylfreeradicals(*OH)isconsideredtobeaprerequisitetoanodicO-transferreactionsataPbO2electrode.Inthiswork,amethodwasstudiedbymeansofhigh-performanceliquidchromatography(HPLC)combinedwithelectrochemicaldetection(ED)soastoinvestigatetheproductionofhydroxylfreeradicals(*OH)intheprocessoftheanodicdischargeofH2OataPbO2electrode.ThevoltammetricdataobtainedatthePbO2electrodefortheoxidationofsalicylicacidtosalicylatehydroxylationproducts(DHBAs)andthedetectionofDHBAsbymeansofHPLC-EDconfirmtheproposedmechanism.
简介:Haloaceticacids(HAAs)areformedduringthechlorinationofdrinkingwater,whichareharmfultopeople′shealthduetotheircarcinogenicandmutageniceffects.Inthepresentstudy,adetectionmethodcombiningmethyltert-butylether(MtBE)extractionwithacidcatalysisandgaschromatographycoupledwithanelectroncapturedetector(GC/ECD)wasdevelopedfordeterminingHAAs.Thedetectionlimitofthismethod(MDL)andrelativestandarddeviation(RSD)werebelow0.37μg/Land6.2%,respectively.Thelaboratorychlorinationexperimentswereconductedwiththepurposeofinvestigatingtheinfluencesofreactiontime,temperature,UV254,bromideandammonia-nitrogenontheformationofHAAs.TheresultsshowthattheformationamountofHAAsincreaseswithincreasingreactiontimeandtemperature,respectively;andthereexistsalinearrelationshipbetweentheformationofHAAsandUV254.TheformationamountofHAAsdecreasesfirstandthenincreasesasthebromideionconcentrationincreases,andaddingNH+4isapossiblewaytocontroltheformationofHAAs.
简介:Inconsiderationoftheadsorptionofsolvent,diluentandsolutemoleculesonthesurfaceofastationaryphase,anewequationforsoluteretentioninliquidchromatographyispresented.Thisequationincludesthreeparameters:thedisplacementequilibriumconstant(Ksd)betweenthesolventanddiluentmoleculesonthesurfaceofthestationaryphase,thetotalnumber(N)ofthesolventanddiluentmoleculesreleasedfromthestationaryphaseafteronesolutemoleculebeingadsorbed,andtheparameter(I)relatedtothethermodynamicequilibriumconstantforthesoluteadsorptiononthestationaryphase.Overthewholeconcentrationrangeofthesolventinthemobilephase,theexperimentalretentiondatacanbewelldescribedbythisequation,parametersKsd,NandIcanbeobtainedbytheregressionanalysisoftheexperimentalretentiondata,andconsequentlythenumberofthesolventandthediluentmoleculesdisplacedbyonesolutemoleculefromthestationaryphasecanalsobederivedatdifferentsolventconcentrationsinthemobilephase.