简介:Endogenousaspergillusendophthalmitis(EAE)afterkidneytransplantisararebutimportantclinicalproblemduetopotentiallydevastatingconsequences.EarlydiagnosisofEAE,timelyremovalofaffectedvitreousbyvitrectomy,properanti-fungaltreatment,allcontributedtothesuccessfulcontrolofthedisease.TherapeuticsuccessofEAEinpost-transplantpatientsdependslargelyonpromptdiagnosis.DefinitediagnosisofEAEisbasedonpositivecultureresultsofvitreousspecimen,whilefundoscopyandBscanultrasoundmayaidearlydiagnosis.Intermsofanti-fungalmedicine,amphotericinBhaslongbeenthefirstchoice,butitssystemicapplicaitonhassevereadversereactions,especiallyforpatientswithimpairedrenalfunction.Herein,wereportthetreatmentmodalityofEAEafterkidneytransplantwithvitrectomy,systemicadministrationofmicafunginplusvoriconazole,topicalapplicationoffluconazolandamphotercinB.
简介:目的构建肺炎链球菌自溶素(pneumococcalautolysin,LytA)核酸疫苗,并研究其免疫原性。方法采用聚合酶链反应方法从肺炎链球菌基因组中克隆肺炎链球菌自溶素基因,然后将该基因插入到pVAX1真核表达载体中,构建表达LytA的重组质粒,制备成超螺旋比例大于90%的核酸疫苗。实验组:BALB/c小鼠5只,对其分3次肌内注射核酸疫苗(100μg);对照组:BALB/c小鼠5只,肌内注射pVAX1空载体(100μg)3次。分别采集小鼠血清,采用间接酶联免疫吸附方法测定血清LytA特异性抗体水平。结果扩增出的LytA基因(957bp)与基因库中LytA的编码序列(957bp)一致。成功构建了LytA核酸疫苗。实验组小鼠的血清LytA抗体水平明显高于对照组,差异有统计学意义(P〈0.01)。结论成功克隆了LytA基因,制备了LytA核酸疫苗,后者可在小鼠体内诱导较强的特异性体液免疫反应。
简介:AIM:Toinvestigatetheexpressionofdendriticcell-associatedC-typelectin-1(dectin-1)attheearlyperiodofAspergillusfumigatusinfectioninrat’scornealepithelium.·METHODS:Atotalof72Wistarratswererandomlydividedintothreegroups:A,BandC.Therighteyeswerechosenasexperimentaleyes.GroupAwascontrolgroup.RatsingroupBwerenotinoculatedwithAspergillusfumigatus.GroupCwastakenasAspergillusfumigatuskeratitismodel.RatsingroupBandC(sixfromeachgroup)wereexecutedrandomlyat4,8,16and24hoursafterexperimentalmodelbeingestablishedtoassesstheexpressionofdectin-1mRNAthroughreal-timePCR.AnothersixratsingroupBandCwereexecutedrandomlyat24hourstoassesstheexpressionofdectin-1proteinthroughimmunohistochemistry.·RESULTS:Theresultsofreal-timePCRindicatedthatdectin-1mRNAexpressionwaslowincornealepitheliumofnormalrats’.Therewasnosignificantlydifferenceofdectin-1mRNAexpressioningroupAandB(P>0.05).TheexpressionofAspergillusfumigatusinfectedcornealepitheliumincreasedgraduallyafter8hoursingroupC.ThesynchronousexpressionofgroupAandChadsignificantdifference(P<0.01).Immunohistochemistydiscoveredthatdectin-1receptorexistedinnormalrat’scornealepithelium.Dectin-1proteinincreasedafter24hoursingroupC.TherewasasignificantdifferenceofsynchronousexpressioningroupBandC(P<0.01).·CONCLUSION:Dectin-1existsinrat’scornealepitheliumanditsexpressionsignificantlyincreasesattheearlyperiodofAspergillusfumigatusinfection.Dectin-1isapatternrecognitionreceptorthatexpressesincornealepitheliumandinvolvesinimmuneresponsetoAspergillusfungalkeratitis.
简介:<正>DearSir,IamDr.Sheng-LiMi,fromtheBiomanufacturingengineeringlaboratory,GraduateSchoolatShenzhen,TsinghuaUniversity,Shenzhen,China.Iwritetopresentacasereportofnocardiabrasiliensisinapatientwithdiabetes.Nocardiaispartofagroupofaerobicactinomycetes,widelydistributedinsoil.Nocardiaasteroidsandnocardiabrasiliensisarethemostcommonhumanpathogens.Humansinfectedbynocardiaasteroidsthroughtherespiratorytractmainlysufferfromprimarysuppurativepulmonaryconditions.Infectionofnocardiabrasiliensisoftenoccursintheadvancedstagesofaprogressivediseaseorimmunedisorder,especiallyCushingsyndrome,diabetes,orinpatientsusingcorticosteroids,immunosuppressiveagentsandbroad-spectrumantibioticsforlongtime[1].Toourknowledge,thereportsofnocardiakeratitiswasveryrare