简介：Objective:Toevaluatetheclinicalutilityoftestingserumanti-treponemapallidumIgMantibodyinthediagnosisofsyphilispatients.Methods:Seventy-twocasesofsyphilisweretestedforspecificIgMantibodywithELISA,andtheresultswerecomparedwithRPRandTPPA.Results:ThesensitivityofIgMantibodywas73.3%(11/15)inprimarysyphilis,88.9%(16/18)insec-ondarysyphilis,andtherewasnosignificantdiffer-encebetweenthesevalues(x^2=1.6363,P>0.10).ThesensitivityofIgMantibodyindiagnosinglatentsyphi-liswasonly26.1%(6/23),muchlowerthanthedetec-tionrateinsymptomaticearlvsvDhilis(x^2=17.6189.P<0.005).RPRandTPPAwereboth100%sensitiveinlatentandearlysymptomaticsyphilis.Twowereposi,fiveforIgMinthe16caseswhohadreceivedregulartreatments2to24monthsbeforeenrolled.Conclusions:SpecificIgMantibodydetectiondoeesnotappearsuperiortoRPRandTPPAindiagnosingprimarysyphilis.ThediagnosisoflatentsyphilisshouldmainlyrelyonRPRandTPPA,sincetherearelowtitersofIgMantibodyatthatstage.IgMantibodytestingaloneshouldnotberecommendedformonitor-ingsyphilisdevelopmentortreatmentefficacy.Fur-therstudiesshouldbeconcerned.

简介：Objective:ToevaluatethevalueofenhancedluminescenceenzymeimmunoassayinthediagnosisofNeisseriagonorrhea(NG)infection.Methods:Anti-catalaseantibodyforNeisseriagonorrheaecombinedwithenhancedluminescenceenzymeimmunoassaywereusedtotestforN.gonorrhea.Results:Aminimumof1×10^4/CFUofGCingenitaltractsecretionsorurinecouldbedetectedwiththetechniqueofluminescenceenzymeimmunoassay.Conclusion:TheenhancedluminescenceenzymeimmunoassayhastheadvantageofhighsensitivityandspecificityfordiagnosingNGfromgenitourinarytractsecretionandurine.

简介：

简介：

简介：Objective:Toinvestigatetherolesofinterleukin-2(IL-2)andinterleukin-10(IL-10)inpathogenesisofearlysyphilis.Methods:TheserumlevelsofIL-2andIL-10in48patientswithearlysyphilisweredetectedbyABC-ELISA.Results:(1)ThelevelofIL-2inthepatientswithearlysyphiliswassignificantlyhigherthanthatinhealthycontrols,whilethatofIL-10waslower(P<0.001andP<0.001).(2)ThelevelsofIL-2andIL-10werealmostidenticalinpatientswithprimaryandsecondarysyphilis(P>0.05),aswellasbetweendif-ferentRPRtiters(P>0.05).(3)Aftertherapy,thelevelofIL-2decreasedmarkedly(P<0.05),whilethatofIL-10increase(p>0.05).(4)AsignificantcorrelationwasfoundbetweentheserumlevelsofIL-2andIL-10(r=0.5385P<0.05).Conclusions:Th1up-regulationoccursinpatientswithearlysyphilis,andplaysanactiveroleinfightingagainstTPinfection.

简介：Objective:TostudythecellularimmunityfunctionofpatientswithearlysyphilisandtheeffectsonimmunemodifiersEsberitoxNorIFN.Methods:T-lymphocytesubpopulationsoftheperipheralbloodin44patientswithsyphilisand40healthycontrolswereexaminedbyflowcytometry.Results:ThenumberofCD4+cellsandtheCD4+/CD8+ratioinpatientswithsyphiliswerefoundtobesignificantlylowerthanthoseinthecontrol(P<0.01),whilethenumberofCD8+cellswashigherthanthatinthecontrol(P<0.01).TheCD4+/CD8+ratiointhosewithactivediseasewaslowerthanthatinthosewhohadbeencured(P<0.05).TheCD4+countandtheCD4+/CD8+ratiointhosetreatedwithantibioticsalone(PenicillinGorCephalosporins)werelowerthanthosetreatedwithbothantibioticsandimmunomodulators(P<0.05).Conclusions:Cellularimmunityinthepatientswithearlysyphiliswasprominentlysuppressed,andtreatmentwithimmunomodulatorsmaybehelpfulfortherecoveryofcellularimmunityofthesepatients.

简介：Objectives:Toevaluatetheefficacyofnestedpolymerasechainreaction(PCR)withfirstvoidurine(FVU)forthediagnosisofMycoplasmahominisinmalepatients.Methods:MatchedFVUspecimensandurethralswabswerecollectedfrom194malepatientswithNongonococcalUrethritisandtestedbynestedPCRandcellculture.Cellculturewasusedasagoldstandardforevaluatingotherassaytechniques.Results:ForFVUnestedPCRassayandFVUcellculture,ourresultsshowedthatthesensitivitywas100%and93.3%;specificitywas97.0%and98.2%;positivepredictivevalue(PPV)was85.7%and90.3%,negativepredictivevalue(NPV)was100%and98.8%,respectively.Thetotalconsistencybetweenthetwotechniqueswas97.4%.Conclusions:ForthediagnosisofMycoplasmahominisinmen,nestedPCRdetectingFVUisahighlysensitiveandspecificmethod.FirstvoidurinecanreplaceswabcultureorPCRintermsofacceptabilityandfeasibility.

简介：Objectives:TodevelopamethodofsimultaneousPCRdetectionofHaemophilusducreyi,Treponemapallidum,andHerpesSimplexVirusTypes1and2fromgenitalulcersamongpatientsattendingSTDclinicsinGuangzhou,China;andevaluatetheclinicalapplicationofmultiplexPCR(M-PCR)assayfordiagnosingtheetiologyofgenitalulcerdiseases(GUD).Methods:244patientswithagenitalulcerwereevaluated.ClinicaletiologyofGUDwasbasedonphysicalappearanceandmicrobiologicevaluationsthatincludeddarkfieldmicroscopyexamination(D-F)andserologytestforsyphilis(STS).SwabsofeachgenitalulcerweretestedforHSVantigenbyenzymeimmunoassay(EIA)andprocessedinanM-PCRassayforsimultaneousdetectionofT.pallidum,HSVandH.ducreyi.Results:ThestandardstrainsofT.pallidum,HSVandH.ducreyiwereamplifiedbyM-PCR,producingamplifiedproductsof260bp,432bp,170bp,respectively.ThesensitivityofM-PCRis102pgDNA.M-PCRassayforT.pallidum,HSVandH.ducreyishowedgoodagreementwhencomparedwithD-FdetectionforT.pallidum,STS,H.ducreyicultureandEIAforHSVantigen(Kappascoresare0.774,0.704,0.793,0.756,respectively).Conclusions:TheM-PCRisaconvenient,accurateandreliableassayforthedetectionofT.pallidum,HSVandH.ducreyifromgenitalulcers,andcanbeusedasamethodofdiagnosingtheetiologyofGUD.

简介：Objective:TounderstandthechangeofTh1/Th2andTc1/Tc2balanceinpatientswithsecondaryearlysyphilis.Methods:PeripheralbloodThandTccellsubpopulationswereexaminedbythree-colorflowcytometryin30patientswithsecondaryearlysyphilisand20healthysubjects.Results:PercentagesofCD3^+,CD4^+TcellsandtheratioofCD4^+/CD8^+andNKcellsinsyphiliticpatientsweresignificantlylowerthaninthecontrolgroup(P<0.001),butnostatisticaldifferencewasfoundinpercentagesofCD8^+andCD19^+cellsbetweenthetwogroups(P>0.05).ThepercentageofTh1cellsandratiosofThl/Th2andTcl/Tc2insyphiliticpatientswereclearlydecreasedascomparedtothoseincontrois(P<0.001-0.01),whereaspercentagesofTc0andTc2cellsinsyphiliticswashigherthanthoseincontrols(P<0.001).Conclusion:Therecouldbeadisturbanceofcellularimmunityandtherelativelyintactfunctionofhumoralimmunityinsecondarysyphilis.ItispostulatedthatthepolarizationtowarddevelopmentofTh2andTc2cellsandtheirassociatedcytokinesmightbeinvolvedintheincompleteclearanceoftreponemesandthedevelopmentofchronicinfection.