简介:AcomputerscientistatWashingtonUniversityinSt.Louishasdevelopedawaytocoaxcellstodonaturalthingsunderunnaturalcircumstances,whichcouldbeusefulforstemcellresearch,genetherapyandbiofuelproduction.MichaelBrent,theHenryEdwinSeverProfessorofEngineeringintheSchoolofEngineering&
简介:Thispaperdevelopesatemperaturecontrolsysteminordertocarryoutarealtimeandcontinuousobservationonlivingcellsunderthemicroscope.Inthissystem,transparentindiumtinoxid(ITO)conductivefilmwasusedasaheater,temperaturewasmeasuredbythetemperaturedependenceofresistancebehavioroftheITOfilm,C8051F340single-clupwasusedasthecontrolhardwarecore,andthemodifiedPIDalgorithmandpulsewidthmodulation(PWM)wereadoptedasthekeycontrolsoftwareprograms.Thetemperaturesystemhadasimplestructurewithoutaddedtemperaturesensor,anuniformtemperaturedistributionwithin±1℃ontheITOfilmsurfaceandtemperaturecontrolprecisionof±0.2℃couldbeobtained.ThesystemcouldmaintainagoodgrowthstateforBHK-21livingcellunderthemicroscopefor48h.