简介：ToscreenandidentifytheshortpeptideswithspecificbindingactivitytohumanCDS9andtodesigntheshort-peptideclampagainsttumorescape,thephagedisplaypeptidelibrarycontaining12peptideswasusedtoselectthehighlyexpressedspecificcoalescentpeptideofhumanCD59inCHOcells.Positivephageclonesobtainedafter5roundsofbiopanninganddetectedwithELISAwereobtained,inwhich8ofthemwithhighbindingactivitytohumanCD59weresequenced.The3sequencesthusobtainedshowedhighhomologywitheachandcertainhomologywithsequencewithhumanCD2(PubMed339HGAAENSISPSS),andallcontainedprimarystructureHXAXXXXXXPXX,ofwhichthissequencemaybethemimicconformationalepitopebindingtohumanCD59.Theseresultsinthepresentstudymaybehelpfultodesigntheshort-peptideclampagainsttheactivesitesofCD59ontumorescape.

简介：ToconstructanexpressionvectorcontainingtheE1glycoproteingeneofrubellavirusforthestudyontheeffectofmutationoftheE1geneglycoproteinandtheanalysisofphylogeneticdifferencesofsequences,thegeneencodingtheE1envelopeglycoproteinwasamplifiedfromrubellavirus,JinanstrainJR23,byRT-PCRandligatedintoPMD-18Tvector.TheclonesthatcarriedtheE1genewereidentifiedafteramprselectionandanalysisofrestrictionenzymedigestion.AftersequencingthisgenewasanalyzedbyDanstarandWinstarprograms,andthemapofphylogenetictreewasdrawn.ThecloneofE1glycoproteinwasthusconstructed.ItwasfoundthatthesequencedifferencesbetweenJR23strainandtheTCRBstrainfromJapanandthosebetweenJR23strainandThomasstrainofEnglandwererathersmallwithdifferencevaluesof0.9%and1.2%respectively.YetthosebetweenJR23strainandBRD2strainfromBeijingandthosebetweenJR23strainandXG379strainfromHongKongwerecomparativelylargerwithdifferencevaluesof7.6%and7.3%respectively.ThesequenceofJR23strainwithotherstrainswaslessthan3%excepttheNCstrain(3.7%).ItconcludesthattheconstructionofE1glycoproteingeneoffersanapproachtostudytherelationshipbetweenstructuresandfunctionsofE1geneanditsgeneproducts.Inthephylogenetictree,itshowsthattherearesignificantdifferencesinthesequencesofrubellavirusisolatedinChina,andthismightbehelpfultodevelopaneffectivesubunitvaccine.