简介：TheroleplayedbyDNAinmolecularbiologyisclearlyrecognizedtobevitaltolifeonthisplanet.8-oxo-7,8-dihydro-2deoxyguanosine(=8-OHdG),isprobablythemostimportantproductof"oxidativestress”inDNA.ItsconcentrationinDNAis,infact.aquantitativeanalysisofthedegreeofDNAdamagethatanorganismhasundergone.Duetotheimportanceof8-OHdGofnucleicacidginmutagenesis,carcinogenesisandaging,numerouschemicalandbiologicalinvestigationshavebeenmadeonthissubjectinthepasttime.Kuchinoandco-workershavefoundthat8-OHdGresidueinDNAismisreadingduringtheprocessofDNAreplication.Recently,somereportshavebeenpresentedonhigh8-OHdGlevelsinpatientssufferingfromvariousdiseasessuchaschronichepatitis,Fanconisanemia,diabetesmellitusandHelicobacterpyloriinfections.Asaresult,8-OHdGisausefulmarkerforthestudyofDNAdamagearisingfromreactiveoxygenspeciesandisofgreatsignificanceforcancerresearch.The8-OHdGlevelsinDNAcanhelpunderstandthemechanismofcarcinogensandleadtomoreeffectivetreatmentsformanydifferenttypesofcancer.Forthesereasons,ananalysisof8-OHdGwithquickness,lowcostandhighaccuracyisrequired.

简介：Tetraphenylporphyrin(TPP)isatypicalred-emittingluminogenshowingevidentaggregationcausedquenching(ACQ)effect.Toenhanceitsemissionefficiencyinsolidstate,fourtetraphenylethene(TPE)unitswereattachedtothefourmeso-positionsofTPPcoreviaestergroupthroughafacileandefficientroute.Thederivedcompound(4(TPE-COO)-TPP)emitsredfluorescence(peakat655nm)withagoodquantumefficiency(F)of7.5%,whichismuchhigherthanthatofTPP(Φ~0.1%).Inmolecularaggregateformedintetrahydrofuran(THF)andwatermixtures,4(TPE-COO)-TPPhasarelativehighFof12%.TheevidentlysubduedACQbehaviorcanbeascribedtothepropellershapeandbulkysizeoftheTPEunits,whichpreventtheclosepackingandstrongp-pinteractionofTPPcores.TheloosemolecularpackingandweakinterchromophoreinteractionswerevalidatedbydifferentcharacterizationmethodsincludingUV-visibleabsorption,steadystateandtransientfluorescencespectroscope,X-raydiffractionandscanningelectronicmicroscopeobservations.Itisnotedthat4(TPE-COO)-TPPhasanemissionefficiencyof14.4%indiluteTHFsolution.ThisisduetotheconjugationbreakbetweentheTPPandTPEmoieties,therotationalandvibrationalmotionsofthephenylgroupscannotquenchthefluorescenceof4(TPE-COO)-TPP.

简介：ToanalyzetheantitumorpotentialandmechanismofactionofsimultaneousNewcastlediseasevirus(NDV)hemagglutinin-neuraminidase(HN)andhumaninterleukin18(hIL-18)genetransferinC57BL/6micewithH22hepatoma,themousemodelwithH22hepatomawasestablishedinC57BL/6mice,andtheantitumoreffectsofthecombinedapplicationofNDVHNandhIL-18wereevaluatedinvivo.Theresultsshowthatthegrowthofestablishedtumorsinmiceimmunizedwithadenovirus(Ad)-HNinconjunctionwithAd-hIL-18wassignificantlyinhibitedcomparedwiththatinmiceimmunizedwithAd-HN,Ad-hIL-18alone,ortheemptyvector(Ad-mock).Furthermore,theimmunizationofmicewithAd-HNinconjunctionwithAd-hIL-18elicitedstrongnaturalkilleractivityandH22tumor-specificcytotoxicTlymphocyte(CTL)responsesinvivo.Inaddition,TcellsfromthelymphnodesofmiceimmunizedwithAd-hIL-18orAd-HN+Ad-hIL-18secretedhighlevelsoftheTh1cytokineIL-2andinterferon-γ(IFN-γ),indicatingthattheregressionoftumorcellsisrelatedtoaTh1-typedominantimmuneresponse.TheseresultsdemonstratethatvaccinationwithNDVHNtogetherwithhIL-18maybeanovelandpowerfulstrategyforcancerimmunotherapy.

简介：一荧光灯分核derivate，N-allyl-1-pyrenemethylammoniumhydrochloride(APA+)，被报导与葫芦形成稳定的hostguest建筑群[8]uril(CB[8])，并且这个性质能被利用到确定CB的纯净[8]经由排放滴定。而且，APA+和CB的1:1建筑群[8]能进一步绑甲基viologen(MV2+)，它是广泛地使用的除草剂的主要成分，提供一个好方法检测MV2+，特别在低集中。

简介：Aconcisewhileeffectivemethodforthedeacylationof1,4-diacetyl-2,5-piperazinedioneanditsderivativesissupplied.

简介：AcheapandrecyclableZnO-betazeolitewasusedascatalystforthesynthesisofbenzothiazolederivatives.Thismethodprovidesseveraladvantagessuchasenvironmentalfriendliness,shortreactiontimes,highyields,simplework-upprocedureandcatalystwassuccessfullyreusedforfourcycleswithoutsignificantlossofactivity.

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简介：DirectC—HfunctionalizationofquinolineN-oxideswitharylboronicacidsisachievedusingKMnO_4asthesoleandefficientoxidativesystem.Thismethodprovidesanefficientprotocoltoconstructregioselectively2-arylquinolineN-oxidesviaradicalcross-couplingreactioninmoderatedtogoodyieldsundermildconditions.