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1 个结果
  • 简介:ActivinAisakindofpre-inflammatoryfactorthatbelongstothetransforminggrowthfactor-β(TGF-β)superfamily.ToinvestigatetheeffectandmechanismofactivinAontheactivitiesofmousemacrophages,thesecretionofNOinthesupernatantofculturedmouseperitonealmacrophageswasexaminedbyNOassaykit,andtheexpressionofiNOS,ActRIIAandARIP2mRNAinmouseperitonealmacrophageswasanalyzedbyRT-PCR.TheresultsshowedthatactivinAstimulatedthesecretionofNOandtheexpressionofiNOSmRNAinnon-activatedmousemacrophagesinatime-anddose-dependentmanner.Incontrast,activinAinthesameconcentrationinhibitedthesecretionofNOinLPS-activatedmousemacrophagesinadose-dependentmanner.ActRIIAwashighlyexpressedonmacrophages,andactivinAupregulatedtheActRIIAmRNAexpressioninmacrophages.Anti-ActRIIAantibodycanblockthesecretionofNOfromthemacrophagesstimulatedbyactivinA.Furthermore,RT-PCRanalysisrevealedthatactivinAenhancedtheARIP2mRNAexpressioninmacrophages.TheseresultsindicatedthatActivinAmaybeaweakactivatorcomparedwithLPStomousemacrophages,andactivinAmaymodulatethesecretionofNOthroughActRIIA-ARIP2signalpathwayinmousemacrophages.

  • 标签: 苯丙酸诺龙A 腹膜 巨噬细胞 小鼠 动物实验