In vivo perfusion staining of atherosclerotic lesions and a novel quantification method for lesion size in sequential aortic root sectioning in murine models-中国期刊网

摘要 ObjectivesTodevelopasimple,accurateandreproduciblemethod,whichcombinesmacroandhistopathologicaltechniquesfordeterminingthedegreeoflipiddepositioningeneticallymodifiedmice.MethodTheentireaortasfromC57BL/6,ldlr-/-andapoE-/-micewerestainedwithSudanⅣusingeitherinvivoperfusionortraditionalinvitroenfacestainingtechniques.Histologicalsectionsofaorticrootandheartswereembeddedintissuefreezingmediumandcutwithacryostat,thenstainedwithOilRedO.Thecalculatedaorticrootareabasedontheaorticrootcircumferencewasusedtoreducemeasurementerrors.ResultsTheinvitroenfacestainingcanstainallfat,whichincludetheadventitialtissuearoundaorta.Howevertheinvivoperfusionstainingcanspecificallystainthefattydepositioninsideofaorta.Bothentireaortaandaorticrootsectionstainingshowedthattherewasahighlysignificantincreaseinfattydepositionintheaortasofthegeneticmodifiedmice.Althoughallmicegeneticbackgroundwassame,theapoE-/-micehadlargeratheroscleroticlesionsthanldlr-/-mice.ConclusionsThenewinvivoperfusionmethodismoreaccuratethantheinvitroenfacemethod.Thecombinationofthesemacroandmicroscopictechniquesovercomestheshortcomingsoftheearlierpublishedmethodswhicharegenerallylimitedtothemeasurementoffattyredstainingareasonly,neglectingnon-specificadventitialfatstainingaroundaortaandaorticrootsectiontissuedistortion.

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In vivo perfusion staining of atherosclerotic lesions and a novel quantification method for lesion size in sequential aortic root sectioning in murine models

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In vivo perfusion staining of atherosclerotic lesions and a novel quantification method for lesion size in sequential aortic root sectioning in murine models

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