摘要
WehaverecentlyclonedapathogeninducibleblastresistancegenePi-khfromtheindicaricelineTetepusingapositionalcloningapproach.Inthisstudy,wecarriedoutstructuralorganizationanalysisofthePi-khlocusinbothindicaandjaponicaricelines.A100kbregioncontaining50kbupstreamand50kbdown-streamsequencesflankingtothePi-khlocuswasselectedfortheinvestigation.Atotalof16genesinindicaand15genesinjaponicawerepredictedandanno-tatedinthisregion.TheaverageGCcontentofindicaandjaponicagenesinthisregionwas53.15%and49.3%,respectively.Bothindicaandjaponicasequenceswerepolymorphicforsimplesequencerepeatshavingmono-,di-,tri-,tetra-,andpentanucleotides.SequenceanalysisofthespecificblastresistantPi-khalleleofTetepandthesusceptiblePi-khalleleofthejaponicaricelineNipponbareshoweddifferencesinthenumberanddistributionofmotifsinvolvedinphosphorylation,resultingintheresistancephenotypeinTetep.
出版日期
2007年01月11日(中国期刊网平台首次上网日期,不代表论文的发表时间)