Tumor Antigen Specific Activation of Primary Human T-Cells Expressing a Virally Encoded Chimeric T-Cell Receptor Specific for p185HER2-中国期刊网

摘要 WehavedevelopedandtestedchimericT-cellreceptors(TCR)specificforp185HER2.Intheseexperiments,retroviralvectorsexpressingtheN297orN29ξreceptorswereconstructedinpRET6.AmphotropicviralproducercellswereestablishedintheGALV-basedPG13packagingcellline.Ficollpurifiedhumanperipheralbloodlymphocytes(PBL)werevitallytransducedusinganoptimizedprotocolincorporatingactivationwithimmobilizedanti-CD3/anti-CD28monoclonalantibodies,followedbyviralinfectioninthepresenceoffibronectinfragmentCH296.Transducedcellswereco-culturedwithhumantumorcelllinesthatoverexpress(SK-OV-3)orunderexpress(MCF7)p185HER2toassayforantigenspecificimmuneresponses.BothCD4^+andCD8^+T-cellstransducedwiththeN297orN29ξchTCRdemonstratedHER2-specificantigenresponses,asdeterminedbyreleaseofTh1likecytokines,andcellularcytotoxicityassays.OurresultssupportthefeasibilityofadoptiveimmunothempywithgeneticallymodifiedT-cellsexpressingachTCRspecificforp185HER2.

1JIAN MIN YANG;MICHAEL S FRIEDMAN;MARIANNE T HUBEN;JENNIFER FULLER;QIAO LI;ALFRED E CHANG;JAMES J MULE;KEVIN T MCDONAGH. In vivo anti-tumor activity of murine hematopoietic stem cells expressing a p185HER2-specific chimeric T-cell receptor gene.基础医学,2006-02.
2邱丙森;王平;高红阳;尚易非;许良中. T-CELL RECEPTOR GENE REARRANGEMENT ANALYSIS IN THE PRIMARY CUTANEOUS T-CELL LYMPHOMA.肿瘤,1997-03.
3Dang W u;Pin Wu;Fuming Qiu;Qichun Wei;Jian Huang. Human γδT-cell subsets and their involvement in tumor mmunity.免疫学,2017-03.
4Zheng Xiao-Hong,Zhang Xi-You,Dong Qian-Qian,Chen Feng,Yang Shou-Bo,Li Wen-Bin. Efficacy and safety of chimeric antigen receptor-T cells in the treatment of B cell lymphoma: a systematic review and meta-analysis.医药卫生,2020-08.
5Heng Mei,Fangping Chen,Yue Han,Ming Hou,He Huang,Xiaojun Huang,Yuhua Li,Aibin Liang,Qifa Liu,Ting Niu,Jun Peng,Wenbin Qian,Yongping Song,Jianxiang Wang,Ying Wang,Depei Wu,Kailin Xu,Linhua Yang,Renchi Yang,Lei Zhang,Liansheng Zhang,Xi Zhang,Xiaohui Zhang,Weili Zhao,Weidong Han,Yu Hu. Chinese expert consensus on the management of chimeric antigen receptor T cell therapy-associated coagulopathy.医药卫生,2022-12.
6Hao Lin,Jie Zhang,Shaobo Dong,Yaning Liu,Peipei Liu,George F. Gao,William J. Liu,Guizhen Wu. An adjusted ELISpot-based immunoassay for evaluation of SARS-CoV-2-specific T-cell responses.医药卫生,2022-12.
7Zhang Xian,Li Jing-Jing,Lu Pei-Hua. Advances in the development of chimeric antigen receptor-T-cell therapy in B-cell acute lymphoblastic leukemia.医药卫生,2020-08.
8Yun Chen;Hua Sun;Genyan Liu;Bing Wang;Fang Wang;Beicheng Sun;Kun Yao. EBV LMP2A-specific T Cell Immune Responses Elicited by Dendritic Cells Loaded with LMP2A Protein.免疫学,2009-04.
9Hongliang Fang;Bing Ang;Xinyun Xu;Xiaohui Huang;Yanfeng Wu;Yanping Sun;Wenying Wang;Nan Li;Xuetao Cao;Tao Wan. TLR4 is essential for dendritic cell activation and anti-tumor T-cell response enhancement by DAMPs released from chemically stressed cancer cells.免疫学,2014-02.
10Doerthe Kuester;Michael Vieth;Ulrich Peitz;Stefan Kahl;Manfred Stolte;Albert Roessner;Ekkehard Weber;Peter Malfertheiner;Thomas Wex. Upregulation of cathepsin W-expressing T cells is specific for autoimmune atrophic gastritis compared to other types of chronic gastritis.消化系统,2005-12.

Tumor Antigen Specific Activation of Primary Human T-Cells Expressing a Virally Encoded Chimeric T-Cell Receptor Specific for p185HER2

来源期刊

相关推荐

同分类资源更多

相关关键词

Tumor Antigen Specific Activation of Primary Human T-Cells Expressing a Virally Encoded Chimeric T-Cell Receptor Specific for p185HER2

来源期刊

相关推荐

同分类资源 更多

相关关键词

同分类资源更多