摘要
AIM:TOinvestigatetheimmunogenicityofcandidateDNAvaccineagainsthepatitisCvirus(HCV)deliveredbytwoplasmidsexpressingHCVenvelopeprotein1(El)andenvelopeprotein2(E2)antigensrespectivelyandtostudytheeffectofCpGadjuvantonthiscandidatevaccine.METHODS:RecombinantplasmJdsexpressingHCVEIandE2antigensrespectivelywereusedtosimultaneouslyinoculatemicewithorwithoutCpGadjuvant.AntiserawerethencollectedandtJtersofantJ-HCVantibodieswereanalyzedbyELISA.Onemonthafterthelastinjection,animalsweresacrificedtopreparesingle-cellsuspensionofsplenocytes.ThesecellsweresubjectedtoHCVantigenspecificproliferaionassaysandcytokinesecretionassaystoevaluatethecellularimmuneresponsesofthevaccinatedanimals.RESULTS:AntibodyresponsestoHCVEIandE2antigensweredetectedinvaccinatedanimals.AnimalsreceivingCpGadjuvanthadslightlylowertitersofanti-HCVantibodiesinthesera,whilethesplenocytesfromtheseanimalsshowedhigherHCV-antigenspecificproliferation.Analysisofcytokinesecretionfromthesplenocyteswasconsistentwiththeaboveresults.Whilenoantigen-specificIL-4secretionwasdetectedforallvaccinatedanimals,HCVantigen-specificINF-γ,secretionwasdetectedforthesplenocytesofvaccinatedanimals.CpGadjuvantenhancedthesecretionofINF-γ,butdidnotchangetheprofileofIL-4secretion.CONCLUSION:VaccinationofmicewithplasmidsencodingHCVE1andE2antigensinduceshumoralandcellularimmuneresponses.CpGadjuvantsignificantlyenhancesthecellularimmuneresponse.
出版日期
2004年12月22日（中国期刊网平台首次上网日期，不代表论文的发表时间）
