Expression of Intracellular Domain of Epidermal Growth Factor Receptor and Generation of Its Monoclonal Antibody-中国期刊网

摘要 Topreparemonoclonalantibodyspecifictoepidermalgrowthfactorreceptor(EGFR)intracellulardomain,itsgenewasamplifiedfromtotalRNAofA431cellbyRT-PCR.ThenthegenewasclonedintoprokaryoticvectorpET30a(+).TherecombinantplasmidwastransformedintoE.ColiBL21(DE3)strainforproteinexpression.RecombinantproteinwasinducedwithIPTGandpurifiedusingNi2+-NTAagarose.Thentheanti-EGFRmonoclonalantibody(nAb)waspreparedwithclassicalhybridomatechnique.Positivecloneswereselectedusingindirectenzyme-linkedinmunoabsorbentassay(ELISA).Totally4hybridomacloneswereobtainedandthesemAbswereIgG1(3clones)andIgG2a(1clone),respectively.Theirlightchainswereallkappachains.WesternblottinganalysisandconfocalimmunofluorescenceassaysdemonstratedthatmAbscouldspecificallyrecognizeEGFRexpressingonA431carcinomacellline.ThemAbswillbeusefulinthestudyofEGFR-mediatedsignaltransduction.

1俞小虹;程浩;郑伟. Expression of Activated Epidermal Growth Factor Receptor and Transcription Factor E2F in Condyloma Accuminata.皮肤病学与性病学,2003-01.
2赖西南;王正国;魏立;王丽丽. Effect of substance P released from peripheral nerve ending on endogenous expression of epidermal growth factor and its receptor in wound healing.临床医学,2002-03.
3初同伟;刘玉刚;王正国;朱佩芳;刘大维. Vascular endothelial growth factor and its receptor expression during the process of fracture healing.临床医学,2008-03.
4Chen-Yuan Gong;Bin Lu;Qian-Wen Hu;Li-Li Ji. Streptozotocin induced diabetic retinopathy in rat and the expression of vascular endothelial growth factor and its receptor.眼科,2013-05.
5Maria Bonello;Andrew Harvey Sims;Simon Peter Langdon. Human epidermal growth factor receptor targeted inhibitors for the treatment of ovarian cancer.肿瘤,2018-04.
6Shen Le-Sang,Jin Xiao-Yan,Wang Xu-Meng,Tou Lai-Zhen,Huang Jian. Advances in endocrine and targeted therapy for hormone-receptorpositive, human epidermal growth factor receptor 2-negative advanced breast cancer.医药卫生,2020-08.
7Wu Saizhu Lv Hongsong Zhou Kexiang Sun Fei Ma Rui Zheng Hua Wei Heming Rong Zhiyi. Up and Down Expression of Androgen Receptor, Estrogen Receptor beta and Platelet Derived Growth Factor beta by Testosterone in Aortic Vascular Smooth Muscle Tissues.心血管疾病,2004-02.
8傅重洋;洪光祥;王发斌. Expression of vascular endothelial growth factor and its fetal liver kinase-1 receptor in spinal cord and dorsal root ganglia after neurotomy of sciatic nerve in rats.临床医学,2005-01.
9赖西南;王正国;朱金明;王丽丽. Effect of substance P on gene expression of transforming growth factor β-1 and its receptors in rat＇s fibroblasts.临床医学,2003-06.
10Ying Xu,Changjun Wang,Yali Xu,Bo Pan,Qiang Sun. Changes in the detection of human epidermal growth factor receptor 2 gene (Her-2) status for Her-2 fluorescent in situ hybridization testing.医药卫生,2022-12.

Expression of Intracellular Domain of Epidermal Growth Factor Receptor and Generation of Its Monoclonal Antibody

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Expression of Intracellular Domain of Epidermal Growth Factor Receptor and Generation of Its Monoclonal Antibody

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