摘要
WehavedeliveredviralvectorscontainingeitherChop2fusedwithGFP,Channelrhodopsin-2(ChR2),orHalorhodopsin(HaloR)fusedwithmCherry(toformlightgatedcationchannelsorchloridepumps,respectively),intothedorsalcochlearnucleus(DCN).OnetoeighteenmonthslaterweexaminedtheCNandinferiorcolliculus(IC)forevidenceofvirallytransfectedcellsandprocesses.ProductionofChR2andHaloRwasobservedthroughouttheDCN.Rhodopsinlocalizationwithinneuronswasdetermined,withelongate,fusiformandgiantcellsidentifiedbasedonmorphologyandlocationwithintheDCN.ProductionofChR2andHaloRwasfoundatboththeinjectionsiteaswellasinregionsprojectingtoandfromtheDCN.LightdrivenneuronalactivityintheDCNwasdependentuponthewavelengthandintensityofthelight,withonlytheappropriatewavelengthresultinginactivationandhigherintensitylightresultinginmoreneuronalactivity.Transfectingcellsviaviraldeliveryofrhodopsinscanbeusefulasatracttracerandasaneuronalmarkertodelineatepathways.Inthefuturerhodopsindeliveryandactivationmaybedevelopedasanalternativetoelectricalstimulationofneurons.
出版日期
2011年01月11日(中国期刊网平台首次上网日期,不代表论文的发表时间)