PROPERTIES AND IMMUNOACTIVITY OF MODIFIED IMMUNOGLOBULING BY METHOXYPOLYETHYLENE GLYCOL-中国期刊网

摘要 Methoxypolyethyleneglycolsof5000dalton(PEG)havebeenattachedcovalentlytohumanserumimmunoglobulinGusing2,4,6-trichloro-s-triazineascouplingagent.Themodi-fiedIgGshowedsubstantialchangesinpropertiesascomparedwiththeunmodifiedIgG.Themod-ifiedIgGshowedincreaseinmolecularweight,increaseoftolerancetotemperatureandPH,de-creaseofimmunogenicityandretainingmostofitsimmunoactivity.TheCDspectrashowednoap-parentchangesinitsmainstructure.IgGwith50.3%ofitsaminogroupsmodifiedbyPEGhaditsimmunogenicitycompletelyre-moved,whilemaintaining53.7%ofitsimmunoactivity.

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PROPERTIES AND IMMUNOACTIVITY OF MODIFIED IMMUNOGLOBULING BY METHOXYPOLYETHYLENE GLYCOL

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PROPERTIES AND IMMUNOACTIVITY OF MODIFIED IMMUNOGLOBULING BY METHOXYPOLYETHYLENE GLYCOL

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