简介：AIM:Toinvestigatetheeffectsofhydrogen-richsaline(HRS)onmicrogliaactivationandSirtuintype1(Sirt1)inratswithN-methyl-N-nitrosourea(MNU)-inducedretinitispigmentosa(RP).METHODS:Ratsweredividedintonorm(N)group,model(M)groupandHRS(H)group.RatsinMandHgroupsweregivensalineandHRSrespectivelypriortoandafteradministrationofMNU.Atoneday(d1)andd3afterwards,electroretinogramandhistologicalexaminationwereperformedtoconfirmtheeffectsofHRSonretinalfunctionandstructureofMNU-inducedRP.Immunofluorescencestainingofanti-ionizedcalcium-bindingadaptermolecule1(Iba1),amakerofmicrogliacells,wasperformed,withquantitativereal-timepolymerasechainreaction(qRT-PCR)foritsmRNAquantification.Moreover,Sirt1mRNAandproteinexpressionintheretinasweredetectedbyWesternblotandqRT-PCR.RESULTS:HRSpreservedtheretinalfunctionandmitigatedthereductionofphotoreceptordegenerationinMNU-treatedretinas.ThepresenceofmicrogliacellswassomewhatmoreobviousinHgroupthanthatinMgroupatd1.HRSsuppressedthefurtheractivationofmicrogliacells,withthenumberofmicrogliacellslessthanthatofMgroupatd3.ResultsofqRT-PCRofIba1wereconsistentwiththoseofimmunofluorescencestaining,withthemRNAexpressionofIba1inHgroupmoreintensivethanthatofMgroupatd1(P<0.05),whilelessthanthatofMgroupatd3(P<0.05).Furthermore,theSirt1mRNAandproteinexpressiondecreasedafterMNUadministration,whileHRSmitigatedtheMNU-induceddownregulationofSirt1.CONCLUSION:HRScaneffectivelykeepmicrogliaactivationinducedbyMNUtoanappropriateextent,whileupregulateSirt1inMNU-inducedRP.

简介：使用连续调幅声信号记录听神经动作电位的方法,测定了豚鼠耳蜗水平的调谐曲线。调幅声的频率特性优于滤波短声及短纯音。调幅声作测试声测定的CAP调谐曲线,均呈锲形,有锐利的尖顶。相隔3天测的CAP调谐曲线形状基本相同,曲线的Q10dB值、CF阀值和CF两边曲线的斜率在两次测试之间十分接近。作者认为:用调幅声作为刺激信号,可记录耳蜗水平的听神经动作电位,由此测得的耳蜗CAP调谐曲线,可为临床听力学进行听觉辨别功能评价提供新的手段。

简介：

简介：AIM:Todeterminetheclinicalfeatures,diagnosisandtreatmentoftheprimarySjogrensyndrome(SS)relatedopticneuritis.METHODS:Theclinicaldataof8patients(12eyes)withprimarySSrelatedopticneuritiswereanalyzedretrospectively.RESULTS:Eightof128consecutivepatientswithopticneuritisresultedfromvariedcausesfulfilledthediagnosticcriteriafortheprimarySS.Theypresentedinitiallywiththesignsandsymptomsofnon-specificopticneuritis,and5patientspresentingwithoutdrynessshowedachronicinflammationofsubmandibularglandorparotidgland,andlymphocyteinfiltrationwasdemonstratedbylabialglandbiopsyin2patients.Therewereserumpositivetitersforanti-SjogrensyndromeA(SSA)in7patientsandanti-SjogrensyndromeB(SSB)in8patients.Anti-aquaporin-4(AQP4)antibodywasnegativeinallthe8patients.Bothglucocorticoidsandimmunosuppressiveagentwereadministered,andvisualacuityelevatedin8eyes(66.7%),3patients(37.5%)recurredinthefollow-up.CONCLUSION:PrimarySSrelatedopticneuritisislesscommonandeasilymisdiagnosed.Theconventionaltherapiesforopticneuritiscouldnotcontroltherecurrence.

简介：Leber'scongenitalamaurosis(LCA)andrecentgenetherapyadvancementfortreatinginheritedretinopathieswereextensiveliteraturereviewedusingMEDLINE,PubMedandEMBASE.Adeno-associatedviralvectorswerethemostutilisedvectorsforoculargenetherapy.Conephotoreceptorcellsmightuseanalternatepathwaywhichwasnotreliantoftheretinalpigmentepithelium(RPE)derivedretinoidisomerohydrolase(RPE65)toaccessthe11-cisretinaldehydechromophore.Researcheffortsdedicatedontheprogressionofagene-basedtherapyforthetreatmentofLCA2.Suchgenetherapyapproacheswereextremelysuccessfulincanine,porcineandrodentLCA2models.TherecombinantAAV2.hRPE65v2adenoassociatedvectorcontainedtheRPE65cDNAandwasreplicationdeficient.ItsinvitroinjectionintargetcellsinducedRPE65proteinproduction.Thegenetherapytrialsthatweresofarconductedforinheritedretinopathieshavegeneratedpromisingresults.PhaseIclinicaltrialstocureLCAandchoroideremiademonstratedthatadeno-associatedviralvectorscontainingRPEgenesandphotoreceptorsrespectively,couldbesuccessfullyadministeredtoinheritedretinopathypatients.AphaseIIItrialispresentlyongoingandifsuccessful,itwillleadthewaytoadditionalgenetherapyattemptstocuremonogenic,inheritedretinopathies.

简介：

简介：~~

简介：Aim:ToverifywhetherpartialintraoperativeTenon'scapsuleresection(PTCR)withadjunctiveMitomycinCiseffectiveindevelopingthin,avascularblebsineyesundergoingAhmedglaucomavalveinsertionandtoassesstheefficacyandsafetyofthisprocedure.Methods:ThisstudywasconductedinfourLatinAmericacountries(Argentina,Brazil,ColombiaandPeru).AhmedglaucomavalveimplantinsertionwithPTCR(groupA)andwithoutPCTR(groupB)wasperformedinneovascular

简介：目的探讨缩短小切口非超乳白内障摘除人工晶体晶植人术学习曲线的途径.方法对2006年萧县彭年光明行动中完成29例小切口非超乳白内障摘除人工晶体晶植入术的病例进行回顾性分析.结果通过最先完成17例小切口非超乳白内障摘除人工晶体晶植入术部分手术操作步骤,循序渐进最后独立完成12例小切口非超乳白内障摘除人工晶体晶植入术的全部操作步骤.结论熟练掌握白内障囊外摘除+人工晶体植入技术,是缩短小切口非超乳白内障摘除+IOL植入学习曲线的关键因素.

简介：目的观察白三烯拮抗剂孟鲁司特对儿童分泌性中耳炎（SOM）声导抗曲线恢复的作用。方法选取门诊急性SOM患儿80例（108耳）,随机分为观察组和对照组,观察组42例（56耳）、对照组38例（52耳）。2组均给予抗生素1周、糠酸莫米松鼻喷雾剂2周及黏液促排剂2周治疗,观察组在此基础上加用白三烯拮抗剂孟鲁司特,疗程2周,观察疗效。结果观察组的有效率（69.6%）明显高于对照组（42.3%）,差异有统计学意义（P〈0.05）。结论在儿童SOM的治疗中加用白三烯拮抗剂有助于声导抗曲线的恢复。

简介：白内障是最常见的致盲眼病之一。谷胱甘肽S转移酶（glutathioneS-transferases,GSTs）是典型的多功能酶,主要催化各种化学剂与谷胱甘肽结合。近些年的研究表明,GSTs有抗过氧化引起的白内障作用。GSTs表达水平的高低在白内障形成中有着重要的作用。我们将近年来GSTs与年龄相关性白内障的研究情况做一综述。

简介：AIMTo在Descemet的家根据厚度评估视觉尖酸和endothelial房间密度剥去自动化endothelialkeratoplasty(DSAEK)在外科以后的年。

简介：