Increased Transendothelial Cells Permeability Induced by Oxidized Lipoprotein(a) Relates to Reactive Oxygen Species Generation and Desmogleins Expression

(整期优先)网络出版时间:2016-03-13
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Objective:Thispaperaimsatmeasurementenhancedeffectofoxidizedlipoprotien(a)[oxLp(a)]onpermeabilityofmonolayerendothelialcellsandrelationshipwithreactiveoxygenspecies(ROS)generationanddesmogleins(DSGs)expression.MethodsandResults:Transendothelialpermeabilitywasassayedbytranswellandreactiveoxygenspecies(ROS)wasdeterminedbyDCFH-DAstaining.RT-PCRwascarriedouttodetermineDSGlandDSC2expressioninmRNA,respectively.TransendothelialpermeabilitywasenhancedbyoxLP(a)doseandtimedependently.Themostmarkedeffectappearedataconcentrationof100mg/L,Transendothelialpermeabilityreachedthemaximumvalueafter2hofFITC-dextranaddition,andthengraduallydecreasedafter4h.oxLp(a)inducesthegenerationofcellularreactiveoxygenspecies(ROS),andthiseffectcouldbeinhibitedbysuperoxidedismutase(SOD).IncubationofHUVECswithoxLp(a)resultedinadoseandtime-dependentdown-regulationofDSGlandDSC2expressionattranscriptionallevel.Conclusion:PermeabilityofmonolayerendothelialcellswasenhancedbyoxLp(a)whichisrelatedtoup-regulatingROSformationanddown-regulatingdesmogleinsexpression.