Irrelevance of BKCa Channel Expression to VSMCs Phenotype under Shear Stress

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摘要 LargeconductanceCa~(2+)-activatedK~+(BK_(Ca))channelexhibitsaphenotype-dependentexpressiononvascularsmoothmusclecells(VSMCs),whichpreferstocontractilephenotype.Meanwhile,shearstressdefinitelyinfluencesVSMCsproliferationandcontraction.Thereby,ahypothesiswasraised,wouldshearstresschangetheBK_(Ca)expressionandcorrelatewithVSMCphenotype?Inordertoinvestigateit,VSMCswereexposedtoshearstressinaparallel-plateflowchamberwith12dynes/cm~2for12h.Subsequently,theeffectofshearstressonVSMCproliferation,BK_(Ca)channelexpressionandcontractilephenotypemarker,α-smoothmusclecellactin(α-SMA)andsmoothmusclemyosinheavychain(SMMHC),wasdeterminedbyimmunofluorescencemicroscopy,flowcytometeryaswellasreversetranscriptionpolymerasechainreaction(RT-PCR),respectively.DatashowthatshearstressenhancedtheexpressionofBK_(Ca)channelwhileinhibitingVSMCproliferation.Paralleledtothosephenomena,theexpressionofbothα-SMAandSM-MHCweredecreasedsignificantly.TheseresultsdemonstratedthatupregulationofBK_(Ca)channelwasirrelevanttothemaintenanceVSMCofcontractilephenotypeundershearstress.ThisfindingprovidesanewinsightintounderstandingthecorrelationofBK_(Ca)channelandVSMCphenotype.
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出版日期 2016年02月12日(中国期刊网平台首次上网日期,不代表论文的发表时间)
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