Isozymes Analysis of the Golden Cuttlefish Sepia esculenta(Cephalopoda : Sepiidae)

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摘要 Thirty-nineisozymesinfourtissues(mantlemuscle,buccalbulbmuscle,eyeandliver)ofSepiaesculentawerescreenedforenzymaticanalysisusingstarchgelelectrophoretictechnique.Eighteenenzymes(G3PDH,LDH,MDH,MEP,IDHP,PGDH,GRS,NP,AAT,CK,AK,EST,ALP,ACP,FBP,MPI,GPIandPGM)showstrongactivitiesandgoodconvergenceinzymogram.TheyareprovedtobesuitablegeneticmarkersinSepiaesculenta.Amongthetissuesused,mantlemuscleisthebestforelectrophoreticanalysisofisozymes.Eyeandliverarefairlygoodforsomespecialenzymes,suchasLDH,EST,MPI,etc.Twenty-sixlociaredetected.Theproportionofpolymorphiclociis0.115intheQingdaosampleand0.153intheRizhaosample(P<0.99).ThemeanvaluesoftheobservedandexpectedheterozygosityperlocusofQingdaosampleare0.016and0.017,whilethoseoftheRizhaosampleare0.023and0.025respectively.
机构地区 不详
出版日期 2004年01月11日(中国期刊网平台首次上网日期,不代表论文的发表时间)
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