Rapid Creation of New Photoperiod-/Thermo-Sensitive Genic Male-Sterile Rice Materials by CRISPR/Cas9 System-中国期刊网

摘要 RiceisanimportantfoodcropinChina,andthedevelopmentofhybridriceisacrucialwaytoincreasegrainyield.Thecreationofdual-purposenuclear-sterilelinesfortwo-linehybridbreedinghasbecomevitalforcommercialricebreeding.WeconstructedthepC1300-2x35S::Cas9-sgRNAPTGMS2-1expressionvectorforeditingthemalefertilitygenePTGMS2-1intwowidelycompatiblericevarieties,93-11andHuazhan,byusingtheCRISPR/Cas9system.Weobtainedthemarker-freephotoperiod-/thermo-sensitivegenicmale-sterile(P/TGMS)linesinT1generation.Accordingtotheexperimentsinphytotronwithfourtemperatureandphotoperiodtreatments,wefoundthetemperatureisthemainfactorforrestoringthepollenfertilityofptgms2-1mutantsin93-11andHuazhan,andthephotoperiodalsohassomeeffectsonpollenfertilityintwodifferentricebackgrounds.Theapplicationofcultivatingnewmale-sterilelinesbygenomeeditingsystemwillsignificantlyacceleratethericebreedingprocess.

1CHEN Li—yun XIAO Ying-hui LEI Dong-yang. Mechanism of Sterility and Breeding Strategies for Photoperiod/Thermo-Sensitive Genic Male Sterile Rice.作物学,2010-03.
2LU Chuan-gen;XIA Shi-jian;CHEN Jing;Hu Ning;YAO Ke-min. Plant Temperature and Its Simulation Model of Thermo-Sensitive Genic Male Sterile Rice.作物学,2008-03.
3HEHao-hua HUANGWen-xin PENGXiao-song ZHUChang-lan LIUYi-bai. Inheritance of the Male Sterility in a New Photo／Thermo-Sensitive Genic Male Sterile Line B06S of Rice.作物学,2004-04.
4LIANG Wei-hong;WU Nai-hu. Isolation and Analysis of the Promoter of OsRacD from Photoperiod Sensitive Genic Male Sterile Rice.作物学,2006-01.
5TANEE Sreewongchai;WEERACHAI Matthayatthaworn;CHALERMPOL Phumichai;PRAPA Sripichitt. Introgression of Gene for Non-Pollen Type Thermo-Sensitive Genic Male Sterility to Thai Rice Cultivars.作物学,2014-02.
6Ying-Qian Peng;Luo-Sheng Tang;Shigeo Yoshida;Ye-Di Zhou. Applications of CRISPR/Cas9 in retinal degenerative diseases.眼科,2017-04.
7Yuquan Zhang,Ziyue Li,Julien Milon Essola,Kun Ge,Xuyan Dai,Huining He,Haihua Xiao,Yuhua Weng,Yuanyu Huang. Biosafety materials: Ushering in a new era of infectious disease diagnosis and treatment with the CRISPR/Cas system.医药卫生,2022-12.
8秦倩,孙文靖. 不断改进的CRISPR/Cas9基因编辑系统.医药卫生,2020-11.
9张天则. CRISPR/Cas9脱靶检测方法的原理分析及对比.医药卫生,2019-02.
10LI Hao;QIN Ruiying;LIU Xiaoshuang;LIAO Shengxiang;XU Rongfang;YANG Jianbo;WEI Pengcheng. CRISPR/Cas9-Mediated Adenine Base Editing in Rice Genome.作物学,2019-02.

Rapid Creation of New Photoperiod-/Thermo-Sensitive Genic Male-Sterile Rice Materials by CRISPR/Cas9 System

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Rapid Creation of New Photoperiod-/Thermo-Sensitive Genic Male-Sterile Rice Materials by CRISPR/Cas9 System

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