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CRISPR/Cas9-Mediated Adenine Base Editing in Rice Genome

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摘要 Precisebaseeditingishighlydesiredinplantfunctionalgenomicresearchandcropmolecularbreeding.Inthisstudy,weconstructedarice-codonoptimizedadeninebaseeditor(ABE)-nCas9toolthatinducedtargetedA·TtoG·Cpointmutationofakeysinglenucleotidepolymorphismsiteinanimportantagriculturalgene.Combinedwiththemodifiedsingle-guideRNAvariant,ourplantABEtoolcanefficientlyachieveadeninebaseeditinginthericegenome.

DOI ojn5n8zljr/1983361

作者 LI Hao;QIN Ruiying;LIU Xiaoshuang;LIAO Shengxiang;XU Rongfang;YANG Jianbo;WEI Pengcheng

机构地区不详

出处《水稻科学：英文版》 2019年2期

关键词 PRECISE optimized ADENINE AGRICULTURAL GENE

分类 [农业科学][作物学]

出版日期 2019年02月12日（中国期刊网平台首次上网日期，不代表论文的发表时间）

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来源期刊

水稻科学：英文版

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相关关键词

PRECISE optimized ADENINE AGRICULTURAL GENE

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