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30 个结果
  • 简介:TheexpressionpatternsofOsPIL11,oneofsixputativephytochrome-interactingfactors,wereanalyzedindifferentorgansoftransgenictobacco(Nicotianatabacum).TheexpressionofOsPIL11wasorgan-specificandwasregulatedbyleafdevelopment,abscisicacid(ABA),jasmonicacid(JA)andsalicylicacid(SA).TofurtherexploretheroleofOsPIL11inplantlightsignaltransduction,aplantexpressionvectorofOsPIL11wasconstructedandintroducedintotobacco.Whengrownundercontinuousredlight,OsPIL11-overexpressedtransgenictobaccoexhibitedshorterhypocotylsandlargercotyledonsandleavescomparedtowild-typeseedlings.Whengrownundercontinuousfar-redlight,however,transgenicandwild-typeseedlingsshowedsimilarphenotypes.TheseresultsindicatethatOsPIL11isinvolvedinredlightinducedde-etiolation,butnotinfar-redlightinducedde-etiolationintransgenictobacco,whichlaysthefoundationfordissectingthefunctionofOsPIL11inphytochrome-mediatedlightsignaltransductioninrice.

  • 标签: RICE phytochrome-interacting factor transgenic tobacco LIGHT
  • 简介:向光性是植物为捕捉更多光能而建立起来的对不良光照条件的适应机制之一.蓝光被证明是引起植物向光性最有效的光源.最近的研究进一步揭示植物体内一定存在一种对蓝光具特殊敏感性从而引起向光性反应的光受体.光生物学家已克隆得到数种cDNA并证明它们当中至少有一种对向光性受体负责,遗憾的是至今并没有弄清楚到底是哪一种cDNA表达向光性受体.就诱导向光性反应而言,红光是无效的,但早在本世纪50年代就已有人证明红光对植物向光性行为产生深刻影响.最新的研究(Liu&Iino1996)已证明红光对蓝光所诱导向光性的影响是由光敏色素这一红光/远红光受体参与调节的.研究发现对蓝光和远红光敏感的向光性受由对红光敏感的光敏色素的严格控制.绝对黑暗条件下生长的植物向光性蓝光刺激无反应,不表现向光性.这样的植物只有接受了红光或远红光前处理后才对向光性蓝光刺激有反应.这一重大发现揭示了向光性反应中光敏色素与向光性受体的相互作用,并为光生物学家彻底搞清楚植物向光性受体提供了一条全新的研究思路.本文就是在这一背景基础上总结了近几年来有关光敏色素和向光性方面研究的新进展.我们希望通过这样的工作能使有关植物向光性受体和植物体内吸收不同波长光的光受体?

  • 标签: 向光性 (植物)避荫 光敏色素 光敏色素突变体 光敏色素A 光敏色素B
  • 简介:这贡献考察Arima和Iachello的交往的波色子模型的对称性质。当动态对称的概念是时现在熟悉的,这未必那么为部分动态对称和伪的扩大观点是动态对称,它能漂亮地在交往的波色子模型的上下文被说明。分析的主要结论是动态对称是少见的当时他们的部分并且伪延期是无所不在的。

  • 标签: 对称性质 波色子 交往 模特儿 ARIMA 子模型
  • 简介:在米饭的Phytochromes被由三个成员,PHYA,PHYB,和PHYC组成的一个基因家庭编码。通过以photomorphogenesis描绘phytochrome异种和野类型(WT),单个phytochromes的角色preliminarily在调整米饭de青白化,flowering时间和富饶被探索了。然而,很少信息都没关于是否或怎么被报导phytochromes在米饭影响叶绿素生合成和叶绿体开发。在这研究,我们比较了野类型和phyA的叶绿素内容,在任何一个白人光(WL)下面成年或红的phyB和phyAphyB异种点亮(R)。结果建议phyB察觉R断然调整叶绿素生合成,当phyA的角色能仅仅在phyB缺乏的异种被检测时。涉及叶绿素生合成的基因的表示层次的分析表明phytochromes由调整protochlorophylloxidoreductaseA(PORA)影响了叶绿素生合成表示。在叶绿体开发的phyB的角色也被分析,并且结果建议phyB察觉R由影响叶绿体和grana的数字调整叶绿体开发,以及叶绿体膜系统。

  • 标签: 叶绿体发育 叶绿素合成 光敏色素 色素突变体 基因家族 生物合成
  • 简介:Inthispaperaviscous-inviscidinteractingflowtheory(IFT)isdevelopedforanincompressible,two-dimensionallaminarflow.IFT’smainpointsareasfollows.(1)Byintroducingaconceptofinteractionlay-erwherethenormalmomentumexchangeisdominating,anewthreelayerstructureisestablished.(2)Throughtheconventionalmanipulationsandbyintroducinganinteractionmodel,boththestreamwiseandnormallengthscalesareprovedtobefunctionsofasingleparameterm,whichisrelatedtothestreamwisepressuregradientandReynoldsnumber.(3)Theapproximateequationsgoverningtheflowofeachlayeraswellasthewholeinteractionflowarederived.ThepresentIFTisapplicabletobothattachedandattached-separationbubble-reattachedflows,TheclassicalboundarylayertheoryandTriple-decktheoryareshowntobetwospecialcasesofthepresenttheoryunderm=0and1/4,respectively.FurthermoreIFTprovidesnewdistinctionsofboththenormalandstreamwiselengthscalesforflow-fieldnumericalcomputationandalsogivesanewapproachtodevelopingthesimpli-fiedNavier-Stokes(SNS)equations.

  • 标签: fluid MECHANICS VISCOUS FLOW separation FLOW
  • 简介:象在旋转液体的范围和磁盘一样的身体的相互作用导致新奇流动结构。在环绕的方向的主要打漩流动被一个第二等的运动在南方的飞机附加。流动被在轴的中心通过一个洞介绍墨水设想并且在在交往的身体之间的中央飞机放射状地散布了。流动结构取决于身体,他们的几何安排和旋转速度给的雷纳兹数字的形状。观察流动结构与PIV测量和数字模拟产生了进一步的调查。关键词旋转液体-模式形成-再通行地区-交往的身体-流动可视化CLC数字O351.3

  • 标签: 旋转流度 模式群 再循环地域 流动可视化
  • 简介:这份报纸为调遣目标追踪学习适应格子和模糊交往的多重模型(AGFIMM)的算法,当集中于固定结构的问题时,不高的多重模型(FSMM)算法费用效率比率和Markov转移交往的概率是困难的确切决定的多重模型(IMM)算法。这个算法认识到适应模型由适应格子调整设定,并且获得由模糊逻辑推理在模型集合匹配度的每个模型。模拟结果证明AGFIMM算法能有效地改进精确性和多重模型算法的费用效率比率,并且对设计应用作为结果合适。

  • 标签: 模糊逻辑推理 交互多模型 模型自适应 网格算法 IMM算法 机动目标跟踪
  • 简介:AZener-Strohcrackinteractingwithanedgedislocationisstudied.Thecrackfacesareassumedtobetractionfree.Theapplied'generalizedloading'forcrackistheinitialdisplacementjumpandtheinterventionoftheedgedislocation.Throughdecomposingtheproblemintotwosubsimpleproblems,usingthesuperpositionprinciple,itssolutionisobtained.Todemonstrateboththevalidityofthesolutionanditspotentialapplication,twosimpleexamplesrelatedtothecrackstressintensityfactorsarepre...

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  • 简介:Manynonlinearquantumopticalphysicsphenomenaneedmoreaccuratewavefunctionsandcorrespondingenergyorquasienergylevelstoaccountfor.AnanalyticexpressionofwavefunctionswithcorrespondingenergylevelsforanatomicelectroninteractingwithaphotonfieldispresentedasanexactsolutiontotheSchrSdinger-likeequationinvolvedwithbothatomicCoulombinteractionandelectron-photoninteraction.Thesolutionisanaturalgeneralizationofthequantum-fieldVolkovstatesforanotherwisefreeelectroninteractingwithaphotonfield.ThesolutionshowsthatanN-levelatominlightformstationarystateswithoutextraenergysplittinginadditiontotheFloquetmechanism.Thetreatmentdevelopedherewithcomputingcodescanbeconvenientlytransferredtoquantumopticsinclassical-fieldversionasresearchtoolstobenefitthewholephysicscommunity.

  • 标签: Volkov-Coulomb problem driven N-level atom EXACT
  • 简介:Moleculardynamicssimulationswereperformedtostudytheinteractionbetweenatomichydrogenandsiliconcarbide.Inthepresentstudy,wefocusontheeffectofthesurfacetemperatureonHinteractingwithsiliconcarbide.ThesimulationresultsshowthattheretentionofHatomsinthesampledecreaseslinearlywithincreasingsurfacetemperature.ThedepthprofileanalysisshowsthatthesampleismodifiedbyHbombardment,andthedensityofHatomsisgreaterthanthoseofSiandCatomsneartheinterfaceregionbetweentheH-containingregionandthebulk.However,nearthesurfaceregionthedensitiesofH,SiandCatomsarealmostequivalent.Inthemodifiedlayer,thebondsconsistofSi-CandSi-HandC-H.ThefractionofSi-Cbondsisthegreatest.OnlyafewC-Hbondsarepresent.

  • 标签: 相互作用 SIC 温度效应 分子动力学模拟 MD 表面温度
  • 简介:heterotrimericguanine核苷酸绑定蛋白质(G蛋白质)被表明了各种各样的发信号调停在植物的小径。然而,它在发信号的phytochromeA(phyA)的角色留下逃犯。在这研究,我们发现新调停phyA的显型指明了far-red照耀(FR)preconditioned房间死亡,它仅仅在跟随暴露到白光(WL)的FR-grown幼苗的胚轴发生。房间死亡在G变异的gpa1被减轻,但是与野类型(WT)比较在G变异的agb1加重了,在调停phyA的房间死亡小径的GPA1和AGB1的对抗角色的陈述语气。进一步的调查显示nonphotoconvertibleprotochlorophyllide(Pchlide633)的导致FR的累积,在暴露上产生反应的氧种类(ROS)到WL,为前提FR的房间死亡被要求。而且,ROS主要在叶绿体被检测用荧光灯探查。有趣地,到黑暗成年的幼苗的H2O2的申请导致类似于前提FR的房间死亡的显型。这表明ROS是为房间死亡的一个批评调停人。另外,我们观察到agb1比WT幼苗对H2O2更敏感,显示G蛋白质可以也修改到ROS应力的幼苗的敏感。一起拿这些结果,我们推断G蛋白质可以涉及表明小径调整Arabidopsis胚轴的前提FR的房间死亡的phyA。在phyA位于G蛋白质的参与下面发信号的可能的机制在这研究被讨论。

  • 标签: 细胞死亡 植物色素A 鸟嘌呤 胚轴
  • 简介:Interactingmultiplemodelsisthehotspotintheresearchofmaneuveringtargetmodelsatpresent.AhierarchicalideaisintroducedintoIMMalgorithm.Themethodisthatthewholemodelsareorganizedastwolevelstoco-work,andeachcellmodelisanimproved'current'statisticalmodel.Intheimprovedmodel,akindofnonlinearfuzzymembershipfunctionispresentedtogetoverthelimitationoforiginalmodel,whichcannottrackweakmaneuveringtargetprecisely.Atlast,simulationexperimentsprovetheefficientofthenovelalgorithmcomparedtointeractingmultiplemodelandhierarchicalinteractingmultiplemodelbasedoriginal'current'statisticalmodelintrackingprecision.

  • 标签: 交互多模型算法 “当前”统计模型 基础 机动目标模型 模糊隶属函数 跟踪机动目标
  • 简介:Thispaperpresentsacomputationalmodelforthefluiddynamicsinafracturedductilepipeunderhighpressure.Thepressureprofileinfrontofthecracktip,whichisthedrivingsourceofcrackpropagation,iscomputedusinganonlinearwaveequation.Thesolutioniscoupledwithaonedimensionalchokedflowanalysisbehindthecrack.Thesimulationutilizesahighorderoptimizedprefactoredcompact-finitevolumemethodinspace,andlowdispersionanddissipationRunge-Kuttaintime.Asthepipefracturestherapiddepressurizationtakeplaceinsidethepipeandthepropagationofthecrack-inducedwavesstronglyinfluencestheoutflowdynamics.Consistentwiththeexperimentalobservation,themodelpredictstheexpansionwaveinsidethepipe,andthereflectionandoutflowofthewave.Themodelalsohelpscharacterizethepropagationofthecrackdynamicsandfluidflowsaroundthetipofthecrack.

  • 标签: 流体流动 动力学相互作用 高压管道 传播 动力学计算模型 非线性波动方程
  • 简介:在为光玻璃BK7的擦伤过程的有弹性塑料的转变政体和易碎可锻的转变政体被分析基于赫兹波方程和被魏建议的压力比率理论。为有变量被有限元素模拟的ABAQUS软件基于精力破裂理论模仿的砂砾间隔距离的光玻璃BK7的交往的擦伤进程。为光玻璃BK7的双砂砾交往擦伤测试在DMG超声的70-5上被执行线性,由哪个有限元素模拟的可靠性被验证。细工品的表面形态学被扫描电子显微镜学(SEM)分析,它证明沟的宽度随擦伤深度和砂砾间隔距离的增加显然增加了。沟的宽度的结果与模拟结果一致。表面下的损坏层被蚀刻的HF酸的方法分析,它证明有交叉的裂缝的一个区域。抓的力量被KISTLER的threedimensional测力计测量,它证明第二抓的力量随抓深度和砂砾间隔距离的增加增加了。在第二处擦伤的力量在第一次是比那小的,它与Griffith破裂理论一致。

  • 标签: 砂砾 玻璃 模拟 交往 扫描电子显微镜 表面形态学
  • 简介:Wehaveinvestigatedthelow-lyingcollectivestatesandelectromagnetictransitionsin94Mowithintheframeworkoftheinteractingbosonmodel.Theinfluenceofmodelparametersontheenergylevelsandelectromagneticpropertieshasbeeninvestigated.Theanalysisoftheobtainedresultsandtheparametervaluespredictthatthe23+stateisthelowestmixedsymmetrystatewithpureF=Fmax-1inthisnucleus.Thecalculatedresultspredicatethatthe25+(two-Q-phonon)mixedsymmetrystateisclosedtotheJ=2+at2.870MeVintheexperimentaldata,andthe2.965MeVstateisthelowestmixedsymmetrywithJ=3+.

  • 标签: 核物理 ^94Mo混合对称态 相互作用 玻色子模型
  • 简介:TheexpressionpatternsofOsPIL11,oneofsixputativephytochrome-interactingfactors,wereanalyzedindifferentorgansoftransgenictobacco(Nicotianatabacum).TheexpressionofOsPIL11wasorgan-specificandwasregulatedbyleafdevelopment,abscisicacid(ABA),jasmonicacid(JA)andsalicylicacid(SA).TofurtherexploretheroleofOsPIL11inplantlightsignaltransduction,aplantexpressionvectorofOsPIL11wasconstructedandintroducedintotobacco.Whengrownundercontinuousredlight,OsPIL11-overexpressedtransgenictobaccoexhibitedshorterhypocotylsandlargercotyledonsandleavescomparedtowild-typeseedlings.Whengrownundercontinuousfar-redlight,however,transgenicandwild-typeseedlingsshowedsimilarphenotypes.TheseresultsindicatethatOsPIL11isinvolvedinredlightinducedde-etiolation,butnotinfar-redlightinducedde-etiolationintransgenictobacco,whichlaysthefoundationfordissectingthefunctionofOsPIL11inphytochrome-mediatedlightsignaltransductioninrice.

  • 标签: 光信号转导 表达模式 光敏色素 水稻 转基因烟草 植物表达载体
  • 简介:AbstractBackground:The chaperonin containing t-complex (CCT) proteins play an important role in cell cycle-related protein degradation in yeast and mammals. The role of the chaperonin containing t-complex 4 (CCT4), one subtype of CCT proteins, in the progress of hepatocellular carcinoma (HCC) was not fully elucidated. Here, we aimed to explore the mechanisms of CCT4 in HCC.Methods:In this study, we used the UALCAN platform to analyze the relationship between CCT4 and HCC, and the association of CCT4 with the overall survival (OS) of HCC patients was also analyzed. CCT4 expression in HCC tumor tissues and normal tissues was also determined by western blot (WB) assay. Lentivirus vector was used to knock down the CCT4 expression, and quantitative polymerase chain reaction and WB were used to determine the level of CCT4 in HCC cell lines. Cell counting kit-8 (CCK-8) and 5-ethynyl-2'-deoxyuridine (EdU) assays were used to detect the cell proliferation, and flow cytometry (FCM) was performed to evaluate the effect of CCT4 on the apoptosis of HCC cells. Co-immunoprecipitation (co-IP) assay and WB were used to explore the mechanisms of CCT4 regulating the growth of HCC. Data were calculated from at least three replicate experiments and expressed as mean ± standard deviation. Student’s t test, paired t test, and Kaplan-Meier analysis were used to compare across different groups.Results:We found CCT4 was upregulated in HCC tissues compared with normal tissues, and its high expression was associated with poor prognosis (P < 0.001). CCT4 was significantly increased in HCC tumor tissues compared with normal tissues (0.98 ± 0.12 vs. 0.23 ± 0.05, t = 7.73, P < 0.001). After being transfected with CCT4 short-hairpin RNA (shRNA), CCT4 was decreased in mRNA level and protein level in both Huh7 (mRNA level: 0.41 ± 0.07 vs. 1.01 ± 0.11, t = 8.09, P = 0.001; protein level: 0.61 ± 0.03 vs. 0.93 ± 0.07, t = 7.19, P = 0.002) and Hep3b cells (mRNA level: 0.55 ± 0.11 vs. 1.04 ± 0.15, t = 4.51, P = 0.011; protein level: 0.64 ± 0.10 vs. 0.95 ± 0.08, t = 4.32, P = 0.012). CCK8 assay indicated that CCT4 knockdown inhibited cell proliferation in both Huh7 (OD value of 3 days: 0.60 ± 0.14 vs. 0.97 ± 0.16, t = 3.13, P = 0.036; OD value of 4 days: 1.03 ± 0.07 vs. 1.50 ± 0.12, t = 5.97, P = 0.004) and Hep3b (OD value of 3 days: 0.69 ± 0.14 vs. 1.10 ± 0.11, t = 3.91, P = 0.017; OD value of 4 days: 1.12 ± 0.12 vs. 1.48 ± 0.13, t = 3.55, P = 0.024) cells. EdU assay showed that CCT4 knockdown inhibited the cell proliferation in both Huh7 (EdU positive rate: [31.25 ± 3.41]% vs. [58.72 ± 3.78]%, t = 9.34, P = 0.001) and Hep3b cells (EdU positive rate: [44.13 ± 7.02]% vs. [61.79 ± 3.96]%, t = 3.79, P = 0.019). FCM assay suggested that CCT4 knockdown induced apoptosis in HCC cells (apoptosis rate of Huh7: [9.10 ± 0.80]% vs. [3.66 ± 0.64]%, t = -9.18, P = 0.001; apoptosis rate of Hep3b: [6.69 ± 0.72]% vs. [4.20 ± 0.86]%, t = -3.84, P = 0.018). We also found that CCT4 could regulate anaphase-promoting complex (APC)Cdc20 activity via interacting with Cdc20. Furthermore, CCT4 knockdown induced securin (0.65 ± 0.06 vs. 0.44 ± 0.05, t = -4.69, P = 0.009) and B-cell lymphoma-2 (Bcl-2) interacting mediator of cell death (Bim; 0.96 ± 0.06 vs. 0.61 ± 0.09, t = -5.65, P = 0.005) accumulation. The upregulation of securin inhibited cell growth by downregulating cyclin D1 (0.65 ± 0.05 vs. 1.04 ± 0.07, t = 8.12, P = 0.001), and the accumulation of Bim inhibited Bcl-2 (0.77 ± 0.04 vs. 0.87 ± 0.04, t = 3.00, P = 0.040) and activated caspase 9 (caspase 9: 0.77 ± 0.04 vs. 0.84 ± 0.05, t = 1.81, P = 0.145; cleaved caspase 9: 0.64 ± 0.06 vs. 0.16 ± 0.07, t = 1.81, P = 0.001), which led to elevated apoptosis.Conclusions:Overall, these results showed that CCT4 played an important role in HCC pathogenesis through, at least partly, interacting with Cdc20.

  • 标签: Hepatocellular carcinoma Chaperonin containing t-complex 4 (CCT4) Cdc20 Securin Bim
  • 简介:WithintheframeworkoftheUsdpf(16)interactingbosonmodel(IBM),theeffectsofstrongcorrelationsofthedipole(p--boson)andtheoctupole(f--boson)degreeoffreedomonthepositive-paritystatesofeven-evennucleiinSU(3)limitarediscussed.Itisshownthatconfigurationsofanevennumberofmanyp-andf-bosonscannotonlybeincorporatedintotheusuallow-lyingcollectiverotationalbands,suchasthegroundstateband,β-andγ-vibrationalbands,butalsonaturallyformtheKπ=1+,3+rotationalbands,etc.TheseresultsaresimilartothatofUsdg(15)-IBMandinagreementwellwiththeexperimentaldataofthe17672Hf104nucleus.Besides,severalintrabandE2transitionprobabilitiesaregiven,whichareconsistentwiththatofUsd(6)-IBM.

  • 标签: 玻色子相互作用模型 IBM 偶极矩 强相关性 转动带 原子核物理学