学科分类
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2 个结果
  • 简介:话题在不同表面地里的球形的壳监禁下面的形成柱体的diblock共聚物(DBCP)自己组装用真实空间的前後一致的地理论计算(SCFT)被探索。用这条途径,我们在不同监禁尺寸和表面地观察了形成柱体的DBCP的各种各样的微观结构。从寻找详细说明microdomain形态学,一个明显的结论是在监禁之间的相互作用出现,聚合物穿上大效果自己组装。大多数微观结构大批或在平面、圆柱的监禁下面唯一、不报导。

  • 标签: 嵌段共聚物 圆筒形 球壳 微形态 微观结构 自洽场理论
  • 简介:ThelocalCa^2+releasefromtheheterogeneouslydistributedendoplasmicreticulum(ER)calciumstorehasacriticalroleincalciumhomeostasisandcellularfunction.However;singlefluorescentproteinbasedERcalciumprobesexperiencechallengesinquantifyingtheERcalciumstoreindifferinglivecells,andintensity-basedmeasurementsmakeitdifficulttodetectlocalcalciummicrodomainsintheER.Here,wedevelopedageneticallyencodedratiometricERcalciumindicator[GCEPIA1-SNAPer]thatcandetectthereal-timeERcalciumstoreandlocalcalciummicrodomainsinlivecells.GCEPIA1-SNAPerwaslocatedinthelumenoftheERandshowedalinear;reversibleandrapidresponsetochangesintheERcalciumstore.TheGCEPIA1-SNAPerprobeeffectivelymonitoredthedepletionoftheERcalciumstorebyTGorstarvationtreatment,andthrough让suseweidentifiedheterogeneouslydistributedcalciummicrodomainsintheERwhichwerecorrelatedw让hthedistributionofSTIM1clustersuponERcalciumstoredepletion.Lastly,GCEPIA1-SNAPercanbeusedtodetecttheERcalciumstorebyhigh-throughputflowcytometryandconferstheabilitytostudythefunctionofcalciummicrodomainsoftheER.

  • 标签: RATIOMETRIC CALCIUM sensor LOCAL CALCIUM microdomain