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55 个结果
  • 简介:<正>Fasligand(FasL)wasfirstdescribedfunctionallyasaninduciblecellsurfacemoleculeusedbycytotoxicTcellstoinduceapoptoticcelldeathintumorcellsandactivatedlymphocytes.WiththeidentificationofFasastheIprgeneproduct,FasLbecamerecognizedasamoleculeinvolvedindown-regulationoftheimmunesystem.WhileFasLcanbeusedtoefficientlykillFas-expressingtumorcellsaswellasactivatedTandBlymphocytesinvitro,attemptstouseFasLtherapeuticallytotreatcancerortopreventtransplant

  • 标签: FAS配体 免疫调节作用 毒性 基因治疗 应用
  • 简介:Receptor-ligandinteractionsinbloodflowarecrucialtoinitiatesuchbiologicalprocessesasinflammatorycascade,plateletthrombosis,aswellastumormetastasis.Tomediatecelladhesion,theinteractingreceptorsandligandsmustbeanchoredontotwoapposingsurfacesoftwocellsoracellandasubstratum,i.e.,two-dimensional(2D)binding,whichisdifferentfromthebindingofasolubleligandinfluidphasetoareceptor,i.e.,three-dimensional(3D)binding.Whilenumerousworkshavebeenfocusedon3Dkineticsofreceptor-ligandinteractionsintheimmunesystem,2Dkineticsanditsregulationshavebeenlessunderstood,sincenotheoreticalframeworkorexperimentalassayswereestablisheduntil1993.Notonlydoesthemolecularstructuredominate2Dbindingkinetics,buttheshearforceinbloodflowalsoregulatescelladhesionmediatedbyinteractingreceptorsandligands.Here,weprovideanoverviewofcurrentprogressin2Dbindingandregulations,mainlyfromourgroup.Relevantissuesoftheoreticalframeworks,experimentalmeasurements,kineticratesandbindingaffinities,andforceregulationsarediscussed.

  • 标签: 细胞粘附 相互作用 受体 配体 结合动力学 生物学过程
  • 简介:在血流动的Receptor-ligand相互作用是关键的作为煽动性的串联,血小板血栓,以及肿瘤转移开始生物过程。为了调停,房间粘附,交往的受体和ligands必须被抛锚到二个房间或一个房间和一个基础的二apposing表面上,即,二维(2D)绑定,与在到受体的液体阶段的可溶的ligand的绑定不同,三维(3D)有约束力。当众多的工作在免疫系统集中于receptor-ligand相互作用的3D动力学时,2D动力学和它的规定少些被理解,自从没有理论框架和试验性的试金被建立了直到1993。不仅分子的结构统治在血流动的力量也调整的2D绑定动力学,而是shear房间粘附由交往的受体和ligands调停了。这里,我们在2D绑定和规定提供了当前的进步的概述。理论框架,试验性的大小,运动的率和有约束力的亲密关系的相关问题,和力量规定,被讨论。

  • 标签: 感受器 配合体 动力学 免疫反应 细胞黏附
  • 简介:Themetal-organicchemicalvapordeposition(MOCVD)techniqueisapromisingprocessforhigh-temperaturesuperconductorYBa2Cu3O7-δ(YBCO)preparation.Inthistechnique,itisachallengetoobtainbariumprecursorswithhighvolatility.Inaddition,thepurity,evaporationcharacteristicsandthermostabilityofadoptedprecursorsinthewholeprocesswoulddecidethequalityandreproducibleresultsofYBCOfilm.Inthepresentreport,thebariumprecursorcontaining2,2,6,6-tetramethylheptane-3,5-dionate...

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  • 简介:IncreasedexpressionofFasbyhematopoieticprogenitorsinaplasticanemia(AA)suggeststhatFas/Fasligand(FasL)systemplaysakeyroleintheformationofseverepancytopenia.Tofurtherconfirmtheabovehypothesis,Tcellsfrom8patientswithAAweresystematicallystudiedfortheirFasL'sdistributionpattern,releasingmannerandproapoptoticactivity,comparedwithnormalrestingTcellsandartificiallyactivatedTcellblasts.TheresultsdemonstratedthatAATcellsabnormallyexpressedlowlevelsofmembrane-boundFasLandcontainedhighlevelsofintracellularFasLwhichcouldbetriggeredtoreleasebyhigh-dosephytohemagglutinin(PHA)pulse-stimulation.ThesupernatantsfromthePHA-stimulatedAATcellshadapparentcytotoxicityagainstFasL-sensitiveJurkatcells,whichcouldbesignificantlyinhibitedbymonoclonalantibodyagainstFasLinadose-dependentmanner,ornearlycompletelyabrogatedbyultracentrifugation.TheabovephenomenaalsoappearedonartificiallyactivatedTcellblasts,butthiswasnotthecaseonnormalrestingTcells.TheseresultsindicatethatAATcellisatypeof'preactivated'Tlymphocyte,characterizedbyoverexpressionofFasL,especiallyintracellularFasLwhichcanbestimulatedtoreleaseinbioavtiveexosomesboundform.Takentogether,ourdataprovidefurtheranddirectevidenceforthehypothesisthatTcellsmightmediatethedestructionofhematopieticprogenitorinAAthroughFas/FasLsystem.

  • 标签: 贫血 T-淋巴细胞 免疫表达 免疫反应 免疫细胞
  • 简介:Ahallmarkofallformsofneurodegenerativediseasesisimpairmentofneuronalfunctions,andinmanycasesneuronalcelldeath.Althoughtheetiologyofneurodegenerativediseasesmaybedistinct,differentdiseasesdisplayasimilarpathogenesis,forexampleabnormalimmunitywithinthecentralnervoussystem(CNS),activationofmacrophage/microgliaandtheinvolvementofproinflammatorycytokines.Recentstudiesshowthatneuronsinaneurodegenerativestateundergoahighlyregulatedprogrammedcelldeath,alsocalledapoptosis.TNF-relatedapoptosis-inducingligand(TRAIL),amemberoftheTNFfamily,hasbeenshowntobeinvolvedinapoptosisduringmanydiseases.Asonememberofadeathligandfamily,TRAILwasoriginallythoughttotargetonlytumorcellsandwasnotpresentinCNS.However,recentdatashowedthatTRAILwasunregulatedinHIV-1-infectedandimmune-activatedmacrophages,amajordiseaseinducingcellduringHIV-1-associateddementia(HAD).TRAILisalsoinducedonneuronbyβ-amyloidprotein,animportantpathogenforAlzheimer'sdisease.Inthisreview,wesummarizethepossiblecommonaspectsthatTRAILinvolvedthoseneurodegenerativediseases,TRAILinducedapoptosissignalingintheCNScells,andspecificroleofTRAILinindividualdiseases.Cellular&MolecularImmunology.2005;2(2):113-122.

  • 标签: 白介素 配合基 细胞凋亡 免疫机制 分子免疫 爱滋病
  • 简介:新奇chiral为ligand交换层析(CLEC)的静止阶段(CSP1)被第一把dimethylchlorosilane用作在硅石胶化的表面上为减少的剩余silanol组盖住试剂的结束准备,然后同时作为一个chiral选购者和恐水病的辛基组把l职业人员介绍给硅石胶化表面。CSP1上的14dl氨基的酸的enantioseparations被完成,enantioselectivity和在0.8和6.3之间的决定R从1.09~2.44。有用参考方法准备的结合的阶段(CSP2)的CSP1的色析法的表演被比较。结果证明chiral的列效率和决定R静止阶段能被使用上述修改方法改进。

  • 标签: 氨酰基酯 配体交换色谱法 手征性 光学异构现象
  • 简介:Thedecompositiontemperaturesofthelanthanideorganiccomplexes(η^5-C5H5)2Ln(C6H5CONHO)involvingcyclopentadienylandbenzohydroxamicacidligandswerecalculatedandpredictedbythemodelbasedonANNs(artificialneuralnetowrks)method.ThecomparisonwascarriedoutbetweenresultsfromANNsmethodandtraditinalregressionmethod.ItisprovedthatANNscouldbeusedmoreefficientlyforthepredictionofdecompositiontemperatureoflanthanideorganiccomplexes.

  • 标签: 稀土元素 配合物 人工神经网络 ANNS 结构
  • 简介:BandTlymphocyteattenuator(BTLA),identifiedasanimmuneinhibitoryreceptorrecently,playswidespreadrolesonTandBcells.EmergingevidencehasgeneratedplentifulinformationonthemechanismswhichBTLAmediatesnegativeregulationinimmuneresponsesandinvolvesinavarietyofphysiologicalandpathologicalprocesses.TheexplorationofthebiologicalmechanismsandregulationofBTLAwillopenpossibilitiesonnoveltherapeuticstrategiesinimmune-relateddiseases.

  • 标签: B细胞弱化子 T细胞弱化子 配合体 免疫抑制
  • 简介:Acrylateterpolymer-boundSe,Nbidentateligandwassynthesizedfromthesidechainchlorineofcopolymerandβ-dimethylamino-β′-hydroxyl-diethylselenoether.Thepolymer-supportedplatinumcomplexexhibitedhighcatalyticactivityinthehydrosilylationofolefinswithtriethoxysilane.

  • 标签: 合成 催化活性 铂络合物 双配位基
  • 简介:新不对称的Schiff基础配合体(H_2LLi)是综合usingL离氨酸,水杨酸基醛和2-hydroxy-1-naphthaldehyde。这配合体的三稳固的金属建筑群[行(H_2L)(NO_3)]NO_3·2H_2O(行=La,Sm,惊讶)被元素的分析,红外系列,紫外系列,TG-DTG和臼齿的传导力准备了并且描绘。theligand和它的建筑群的抗菌剂活动也被学习。抗菌剂实验显示它的建筑群拥有的ligandand对Escherichia关口i,葡萄球菌aureus和杆菌的抗菌剂活动敏锐并且建筑群配合体比那些举办更高的活动。

  • 标签: 氨基酸 不对称性 合成工艺 海水化学 抗菌性
  • 简介:TherecyclablePEG-boundbi-cinchonaalkaloidligandhasbeensuccessfullyappliedtothehomogeneouscatalyticasymmetricdihydroxylationofvariousalkenes;goodyieldsandexcellentenantioselectivitieswereobtained,Theligandcouldbeeasilyrecoveredandreusedfor10timeswithoutanyapparentlossofitscatalystefficiency.

  • 标签: 对映选择性 二羟基化作用 烯烃 催化 邻二醇 金鸡纳树
  • 简介:ObjectivesToinvestigatetheassociationofsolubleFasligand(sFasL)andsolubleFasreceptor(sFas)withhumanchroniccongestiveheartfailure(CHF).MethodsTheserumlevelofsFasLandsFasin33patientswithCHF(13incardiacfunctionclassⅡ,17inclassⅢ,3inclassⅣ,NYHA)wasassessedwithenzyme-linkedimmunosorbentassay,andwascomparedwiththatof18age-,bloodpressure-matchedpatientswithcardiacfunctionclassⅠ(NYHA).ResultsTherewasnodifferenceinthelevelofsFasLbetweenthetwogroups[CHFgroup:231.50+/-84.50(cardiacfunctionclassⅡ216.50+/-96.00,classⅢ226.80+/-85.70,classⅣ244.00+/-73.00)vs.cardiacfunctionclassIgroup:217.50+/-89.00pg/mL,P>0.05].However,thelevelofsFaswassignificantlyhigherinthepatientswithCHFthanthoseofcardiacfunctionclassIgroup[CHFgroup:1353.30+/-507.71(cardiacfunctionclassⅡ1154.85+/-371.20,classⅢ1412.88+/-493.62,classⅣ1875.67+/-806.

  • 标签: CONGESTIVE heart failure SOLUBLE FAS LIGAND
  • 简介:Anewanionicdinuclearrutheniumcomplexbeating1,2-bis(diphenylphosphinomethyl)benzene(BDPX)[NH2Et2][{RuCl(BDPX)}2(μ-Cl)3](1)wassynthesizedanditsstructurewasdeterminedbyanX-raycrystallographicanalysis.Thisresultindicatedthatcomplex1consistedofananiondinuclearBDPX-Ruandacationicdiethylarnmonium.Thecrystalbelongedtomonoclinicsystem,C2/cspacegroupwitha=3.3552(7)nm,b=1.8448(4)nm,c=2.4265(5)nm,β=101.89(3)°andZ=8.Thecatalytichydrogenationactivitiesandselectivitiesofcomplex1forcinnamaldehydewereinvestigated,

  • 标签: 双核化合物 钌配合物 催化活性 加氢反应 肉桂醛