学科分类
/ 1
4 个结果
  • 简介:CalciumhydroxytitanateformedinBayerdigestionprocessofchinesediasporicbauxitewasstudied.InterplanarspacingsofcalciumhydroxytitanatecrystalweredeterminedbyXRDanalysis.Onlyoneendothermicpeakoccursat550~560℃ontheDTAcurveofthiscompound.IRspectrometrystudyhasrevealedthathydroxyldoesexistincalciumhydroxytitanateinsteadofcrystalwater.ObservationunderSEMhasindicatedthatitscrystalgrainsareintheshapeofregularhexagonalplateswith2~8μminedgelengthandlessthan1μminthickness.TheinfluencesofvariousdigestionconditionsontheformationandtransformationofcalciumhydroxytitanateandotherCa-Ti-containingcompounds,suchaslimeaddition;contentanddistributionstateofTiO2inbauxite;digestiontemperatureandretentiontime,werefurtherstudied.

  • 标签: DIGESTION retention BAYER HYDROXYL BAUXITE hexagonal
  • 简介:Thepaperanalyzestheinfluenceofleadtoxicitybyanaerobicgranulesludgeinhibitionandrecoveringexperiments.Theresultshowsthattherearedifferentinhibitiontypesatdiffer-entleadcontents.Higherleadcontentleadstomoreinhibitiongranularsludge,andatthesametime,thetimeofgasrecoveryisdifferent.Lowerleadcontentpermicroorganismresultsinsoonersludgerecovery.Microorganismshaveagoodabilitytoresistleadtoxicity.

  • 标签: 厌氧消化 毒理 粒状淤渣 抑制作用 机理
  • 简介:BACKGROUND:Withdevelopmentsoftissueengineeringandgeneticengineering,weaimtoculturemyoblasts,whicharecharacterizedbyhighpurity,highqualityandhighproduction,forwideapplicationinneuralregenerationresearches.OBJECTIVE:Tomodifytraditionaldissociationmethodinordertoobtainmyoblasts,whicharecharacterizedbyhighpurity,highqualityandhighproduction,andexplorethebiologicalpropertiesunderinvitroculture.DESIGN:Observationalstudy.SETTING:BasicInstituteofAcademyofMilitaryMedicalSciencesofChinesePLA.MATERIALS:FourneonatalWistarratsof5daysold,bothgendersandmeanbodymassof10gwereselectedinthisstudy.Themainreagentsanddevicesweredetailedasfollows:DMEMmedium(GibcoCompany),fetusbovineserum(FBS,HycolneCompany),collagenaseⅡ(SigmaCompany),trypsin(SigmaCompany),dispaseⅡ(SigmaCompany),desminantibody(FuzhouMaixinCompany),antibodyⅡandABCkit(WuhanBosterBiotechnologyCompany),deskcentrifuge(KUBATO,Japan),andinvertedphasecontrastmicroscope(LEICADMIRB,Germany).METHODS:TheexperimentwascarriedoutintheBasicInstituteofAcademyofMilitaryMedicalSciencesofChinesePLAfromJunetoOctober2006.Neonatalratsweresacrificedundersterileconditiontoobtainskeletalmusclesoflimbs,whichwerewashedwithcoldPBS(containingbenzylpenicillinandestreptomicina),andmusculartissuewasshearedintopieces.Then,thosemuscularpieceswereaddedwithmixeddigestiveenzyme(containing2g/LcollagenaseⅡ+5g/LdispaseⅡ+0.28g/LCaCl2)astwicevolumeaspieces,dealtwithmechanicalpipettingfor5minutesandculturedinCO2incubatorfor10minutes.Theoperationwasdoneforthreetimesandthemuscularpiecesweredigestedfor45minutesintotal.Moreover,cellsweresuspendedagaininordertoobtainmyoblastsfromskeletalmuscleofneonatalrats.Inaddition,myoblastswerepurifiedwithdifferentialattachmenttechniqueandenzymedigestionsoastoobservemorpho

  • 标签: 大鼠 成肌细胞培养 改良方法 混合酶消化法 双纯化法
  • 简介:Helicoverpaarmigera是在许多重要庄稼的一个关键害虫,它被化学策略主要控制。为了设法,这个害虫对杀虫药剂由于它抵抗的能力和进化正在变得挑战性。进一步,它nonhost植物上的随后的传播在最近的时间是显著的。因此,对phytochemicals和合成杀虫药剂译码抵抗机制是主要挑战。现在的工作描述消化,辩护和豁免联系了酶,这在H与chlorpyrifos抵抗被联系。armigera。H的Proteomic分析。在在用nano液体层析团spectrometry包含饮食(CH)和人工的饮食(广告)的chlorpyrifos上喂之上的armigera内脏织物鉴别在CH的upregulated23蛋白质喂了幼虫。与基因本体论分析相结合的数据库搜索表明识别内脏蛋白质在消化,为免疫关键的蛋白质,强调的适应回答,和detoxification占去。候选人蛋白质的生物化学、量的即时聚合酶链反应分析显示昆虫正在努力在CH的存在得到营养素和精力,当同时努力使chlorpyrifos产生代射变化时。而且,我们在H建议了chlorpyrifos的一条潜在的处理小径。由用煤气的层析团spectrometry检验代谢物的armigera勇气。H。到由调整蛋白质的表示的杀虫药剂的吸引人的行为的、词法改编和电阻的一个范围在消化和detoxification机制包含了处理在上面的armigera展览与chlorpyrifos。在这些上下文,作为内脏是生物信息的一个富有的仓库;内脏纸巾的深刻分析能在昆虫给detoxification和抵抗机制的线索。

  • 标签: 毒死蜱 棉铃虫 杀虫剂 毒蛋白 消化 表达调控