学科分类
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3 个结果
  • 简介:新benzoimidazole-naphthalimide衍生物4被综合,它的photophysical性质被学习。这混合物有选择地高度出现了并且敏感比色并且为氟化物阴离子的ratiometric察觉到能力。

  • 标签: 氟化物 高灵敏比色分析 衍生物 化学试验
  • 简介:ThelocalCa^2+releasefromtheheterogeneouslydistributedendoplasmicreticulum(ER)calciumstorehasacriticalroleincalciumhomeostasisandcellularfunction.However;singlefluorescentproteinbasedERcalciumprobesexperiencechallengesinquantifyingtheERcalciumstoreindifferinglivecells,andintensity-basedmeasurementsmakeitdifficulttodetectlocalcalciummicrodomainsintheER.Here,wedevelopedageneticallyencodedratiometricERcalciumindicator[GCEPIA1-SNAPer]thatcandetectthereal-timeERcalciumstoreandlocalcalciummicrodomainsinlivecells.GCEPIA1-SNAPerwaslocatedinthelumenoftheERandshowedalinear;reversibleandrapidresponsetochangesintheERcalciumstore.TheGCEPIA1-SNAPerprobeeffectivelymonitoredthedepletionoftheERcalciumstorebyTGorstarvationtreatment,andthrough让suseweidentifiedheterogeneouslydistributedcalciummicrodomainsintheERwhichwerecorrelatedw让hthedistributionofSTIM1clustersuponERcalciumstoredepletion.Lastly,GCEPIA1-SNAPercanbeusedtodetecttheERcalciumstorebyhigh-throughputflowcytometryandconferstheabilitytostudythefunctionofcalciummicrodomainsoftheER.

  • 标签: RATIOMETRIC CALCIUM sensor LOCAL CALCIUM microdomain