简介：Differentialproteinsexpressinginectopicandeutopicendometriawereinvestigatedbymeansofproteomicanalysis.Fivepatientsinsecretaryphasewerediagnosedasendometriosisbylaparoscopy.Thefiveectopicendometria(twoatstageⅡ,twoatstageⅢandoneatstageIV)andfiveeutopicendometriaweresurgicallyexcised.One-dimensionalelectrophoresiscoupledwithliquidchromatographyandmassspectrometrywasusedtoscreenandidentifydifferentialproteins.Threedifferentialbandsinone-dimensionalelectrophoresiswereresolvedbyliquidchromatographyandmassspectrometryand14up-regulatedproteinswereidentified,includingcollagenα-1,α-2,α-3(VI),α-1(XIV)chain,actin,annexinA2,EMILIN-1,ferritinlightpolypeptidevariant,fucosyltransferase10,myosin-9,proteinS100-A9,KIAA1783protein,andtwohypotheticalproteins.Ourdataprovidesalistofpotentialbiomarkersforendometriosis.Theidentificationsmaybeusedtodevelopnewdiagnosesforendometriosis.

简介：核是最突出的subnuclear结构，它在真核细胞的细胞的进程执行许多函数。以便在牛的房间理解nucleoli的结构、功能的角色，我们分析了牛的nucleoli的proteomic作文。nucleoli从Madin钞票牛的肾房间被孤立并且由SDS页和强壮的阳离子交换层析在分别以后由LC-MS/MS使分析遭到了到proteomic。用搜索和GPM数据库寻找的吉祥物数据库的数据的分析在牛的nucleoli识别了311蛋白质，它以前包含了22蛋白质没在人的nucleoli的proteomic分析识别。用GoMiner软件的识别蛋白质的分析建议牛的nucleoli包含了涉及ribosomal生物的续生说的蛋白质，房间周期控制，transcriptional，翻译并且translational以后规定，运输，和结构的组织。

简介：Whereasgenomicsdescribesthestudyofgenome,mainlyrepresentedbyitsgeneexpressionontheDNAorRNAlevel,thetermproteomicsdenotesthestudyoftheproteome,whichistheproteincomplementencodedbythegenome.Inrecentyears,thenumberofproteomicexperimentsincreasedtremendously.Whileallfieldsofproteomicshavemademajortechnologicaladvances,thebiggeststepwasseeninbioinformatics.Biologicalinformationmanagementreliesonsequenceandstructuredatabasesandpowerfulsoftwaretoolstotranslateexperimentalresultsintomeaningfulbiologicalhypothesesandanswers.Inthisresourcearticle,IprovideacollectionofdatabasesandsoftwareavailableontheInternetthatareusefultointerpretgenomicandproteomicdata.Thearticleisatoolboxforresearcherswhohavegenomicorproteomicdatasetsandneedtoputtheirfindingsintoabiologicalcontext.

简介：二维的胶化电气泳动(2-DE)由帮助矩阵的激光解吸附作用电离time-of-flight/time-of-flight团spectrometry(MS)列在后面分析习惯于hemolymphproteomic蚕介绍的charaterize，Bombyxmori。在天4(V4)并且5(V5)第五(期末考试)中间形态当幼虫在快成长的阶段时，我们在代表有30kDa的近似分子的重量(MW)的蛋白质的点发现了戏剧的变化。这些点，四30K蛋白质是高度起来调整的，暗示有B的生长和发展的一个靠近的协会。mori幼虫。为了理解分子的基础和内在的机制，在开发和变形包含了，在V5的整个hemolymph的proteome与LTQ-Orbitrap用猎枪液体层析双人脚踏车团spectrometry被分析。没有任何假发现点击，108蛋白质的一个总数被识别。这些蛋白质涉及许多细胞的功能，包括新陈代谢，发展，滋养的运输和储备，并且防卫反应。基因本体论分析证明3.4%这些蛋白质举办了滋养的水库活动，5.7%涉及对刺激的反应。小径分析表明有普通目标的22蛋白质涉及象免疫，区别，增长和变形那样的各种各样的细胞的过程。这些结果建议象在hemolymph的30K蛋白质那样的一些关键因素在B起重要作用。mori生长和开发。而且，hemolymph的多重函数可以被一个复杂生物网络操作。

简介：<正>DearSir,IamProf.BeuyJoobfromSanitation1MedicalAcademicCenter,Bangkok,Thailand.Iwritetodiscusstherecentpublicationonproteomicanalysisindiabeticretinopathy(DR).Liuetal[1]concludedthattheirapproachbytwodimensionalfluorescencedifferencegelelectrophoresis(2D-DIGE)combinedwithmatrix-assistedlaser

简介：精索静脉曲张，男不孕的最重要的可对待的原因，是在场的在15%成年男性，有主要不孕的35%人，和有第二等的不孕的80%人。在另一方面，80%这些人不介绍不孕。因此，有需要区分正在施加从“是可对待的有害效果的精索静脉曲张；silent”；精索静脉曲张。尽管有精索静脉曲张的细胞的效果的成长证据，它的内在的分子的机制仍然正在逃避。Proteomics成为了一个有希望的区域决定精液的繁殖生物学以及改进男不孕的诊断。这评论试图与精索静脉曲张在病人在精液的血浆proteomics讨论state-of-art在承担这些研究，以及在精液的proteome上从证据的成长身体导出的未来眼界讨论挑战。

简介：Inthisstudy,wepresentaconstructivealgorithmfortrainingcooperativesupportvectormachineensembles(CSVMEs).CSVMEcombinesensemblearchitecturedesignwithcooperativetrainingforindividualSVMsinensembles.Unlikemostpreviousstudiesontrainingensembles,CSVMEputsemphasisonbothaccuracyandcollaborationamongindividualSVMsinanensemble.AgroupofSVMsselectedonthebasisofrecursiveclassifiereliminationisusedinCSVME,andthenumberoftheindividualSVMsselectedtoconstructCSVMEisdeterminedby10-foldcross-validation.ThiskindofSVMEhasbeentestedontwoovariancancerdatasetspreviouslyobtainedbyproteomicmassspectrometry.BycombiningseveralindividualSVMs,theproposedmethodachievesbetterperformancethantheSVMEofallbaseSVMs.

简介：Sequence-basedmolecularphylogenieshaveprovidednewmodelsofearlyeukaryoticevolution.Thisincludesthewidelyacceptedhypothesisthatanimalsarerelatedmostcloselytofungi,andthatthetwoshouldbegroupedtogetherastheOpisthokonta.Althoughmostpublishedphylogenieshavesupportedanopisthokontrelationship,anumberofgenescontainatree-buildingsignalthatclustersanimalandgreenplantsequences,totheexclusionoffungi.Thealternativetree-buildingsignalisespeciallyintriguinginlightofemergingdatafromgenomicandproteomicstudiesthatindicatestrikingandpotentiallysynapomorphicsimilaritiesbetweenplantsandanimals.Thispaperreviewsthesenewlinesofevidence,whichhaveyettobeincorporatedintomodelsofbroadscaleeukaryoticevolution.

简介：PlatycodinD(PD)，从Platycodonis根值孤立的triterpenoidsaponin，是被显示了在几根癌症房间线有anti-proliferative效果的一副著名中草药。这研究的目的是用proteomics途径与PD在hepatocellular癌HepG2房间的治疗以后在细胞的蛋白质决定变化。房间生存能力用MTT试金被决定。proteome被二维的差别胶化电气泳动和帮助矩阵的激光解吸附作用/电离time-of-flight团spectrometry分析。西方的污点分析被用来证实改变的蛋白质的表示。我们的结果证明PD在集中依赖者和时间依赖者礼貌禁止了HepG2房间的增长。十六蛋白质被识别在对待PD的HepG2房间起来调整包括ATP5HOXCT1，KRT9，CCDC40，ERP29，RCN1，ZNF175，HNRNPH1，HSP27，PA2G4，哲学学士，BANF1，TPM3，ECH1，LGALS1，和MYL6三蛋白质(即，RPS12，EMG1，和KRT1)与PD在治疗以后在HepG2房间减少了。在HSP27和哲学学士的变化被西方的弄污进一步证实。在结论，我们的结果为PD的反癌症活动在行动的机制上打开新灯。

简介：Insulinsecretorygranules(ISGs),agroupofdistinguishingorganellesinpancreaticβcells,areresponsibleforthestorageandsecretionofinsulintomaintainbloodglucosehomeostasis.ThemolecularmechanismsofISGbiogenesis,maturation,transportation,andexocytosisarestilllargelyunknownbecausetheproteinsinvolvedinthesedistinctstepshavenotbeenfullyidentified.Subcellularfractionationbydensitygradientcentrifugationhasbeensuccessfullyemployedtoanalyzetheproteomesofnumerousorganelles.However,useofthismethodtoelucidatetheISGproteomeislimitedbyco-fractionatedcontaminantsbecause1SGsareverydynamicandhaveabundantexchangesorcontactswithotherorganelles,suchastheGolgiapparatus,lysosomes,andendosomes.Inthisstudy,wedevelopedanewstrategyforidentifyingISGproteinsbyproteincorrelationprofiling(PCP)-basedproteomics,whichincludedISGpurificationbyOptiPrepdensitygradientcentrifugation,label-freequantitativeproteome,andidentificationofISGproteinsbycorrelatingfractionationprofilesbetweencandidatesandknownISGmarkers.Usingthisapproach,wewereabletoidentify81ISGproteins.Amongthem,TM9SF3,anine-transmembraneprotein,wasconsideredahighconfidenceISGcandidateproteinhighlightedinthePCPnetwork.FurtherbiochemicalandimmunofluorescenceassaysindicatedthatTMgSF3localizedinISGs,suggestingthatitisapotentialnewISGmarker.

简介：Objective:Hepatocellularcarcinoma(HCC)isaleadingcauseofcancer-relateddeaths.NovelserumbiomarkersarerequiredtoincreasethesensitivityandspecificityofserumscreeningforearlyHCCdiagnosis.ThisstudyemployedaquantitativeproteomicstrategytoanalyzethedifferentialexpressionofserumglycoproteinsbetweenHCCandnormalcontrolserumsamples.Methods:Lectinaffinitychromatography(LAC)wasusedtoenrichglycoproteinsfromtheserumsamples.Quantitativemassspectrometricanalysiscombinedwithstableisotopedimethyllabelingand2Dliquidchromatography(LC)separationswereperformedtoexaminethedifferentiallevelsofthedetectedproteinsbetweenHCCandcontrolserumsamples.Westernblotwasusedtoanalyzethedifferentialexpressionlevelsofthethreeserumproteins.Results:Atotalof2,280proteingroupswereidentifiedintheserumsamplesfromHCCpatientsbyusingthe2DLC-MS/MSmethod.Upto36proteinswereup-regulatedintheHCCserum,whereas19proteinsweredown-regulated.Threedifferentialglycoproteins,namely,fibrinogengammachain(FGG),FOS-likeantigen2(FOSL2),andα-1,6-mannosylglycoprotein6-β-N-acetylglucosaminyltransferaseB(MGAT5B)werevalidatedbyWesternblot.Allthesethreeproteinswereup-regulatedintheHCCserumsamples.Conclusion:AquantitativeglycoproteomicmethodwasestablishedandprovenusefultodeterminepotentialnovelbiomarkersforHCC.

简介：ASK1(ARABIDOPSIS象SKP1一样)蛋白质是招募的ubiquitinligase建筑群指向的SCF(Skp1-Cullin-F盒子蛋白质)的一个批评部件为由26Sproteosome的降级的蛋白质。为了调查蛋白质，那被调停ASK1的解朊作用小径在Arabidopsis花影响，我们用二维的电气泳动(2-DE)比较了Arabidopsis野类型和ask1异种花芽的proteomes。有在与野类型花相比的ask1异种花的更高或更低的丰富的十个蛋白质点被切除并且使分析遭到了到进一步集体的spectrometry(MS)。结果证明他们是涉及相片形态发生，生理节奏的摆动，翻译以后的过程，压力反应和房间扩大或延伸的蛋白质，建议那些过程在ask1异种被影响。基因也是的这些的抄本层次基于Affymetrix基因芯片比较了微数组数据。没有重要差别为大多数基因被观察，建议有在ask1异种的累积的提高的层次的蛋白质能是一条调停ASK1的解朊作用小径调整的候选人目标。阐明的这些结果帮助在Arabidopsis发展过程并且也的ASK1的多种的功能表明关于基因功能学习蛋白质层次的重要性和必要性。

简介：Brassinosteroid(BR)andgibberellin(GA)aretwogroupsofplantgrowthregulatorsessentialfornormalplantgrowthanddevelopment.TogaininsightintothemolecularmechanismbywhichBRandGAregulatethegrowthanddevelopmentofplants,especiallythemonocotplantrice,itisnecessarytoidentifyandanalyzemoregenesandproteinsthatareregulatedbythem.Withtheavailabilityofdraftsequencesoftwomajortypes,japonicaandindicarice,ithasbecomepossibletoanalyzeexpressionchangesofgenesandproteinsatgenomescale.Inthisreview,wesummarizericefunctionalgenomicresearchbyusingmicroarrayandproteomicapproachesandourrecentresearchresultsfocusingonthecomparisonofcDNAmicroarrayandproteomicanalysesofBR-andGA-regulatedgeneandproteinexpressioninrice.WebelieveourfindingshaveimportantimplicationsforunderstandingthemechanismbywhichBRandGAregulatethegrowthanddevelopmentofrice.

简介：中国白蜡规模昆虫，Ericeruspela，能在-30°C下面在温度在极其低的温度，和某overwintering个人展览supercooling幸存。调查E的深supercooling能力。pela，transcriptomic和proteomic分析被执行描出为overwintering女性的深supercooling能力负责的主要基因和蛋白质家庭。基因本体论(去)基因在表明小径和小径的激活mitogen的蛋白质kinase，钙，和PI3K-Akt包含了，这与可溶的糖，糖白酒和免费氨基酸的生合成联系的分析显示了的基因和染色体(KEGG)的分类和京都百科全书是主导的。为低温度的应力负责的蛋白质例如冷环境适应蛋白质，甘油生合成相关的酶和热吃惊蛋白质(HSP)被识别。然而，防冻剂蛋白质(法新社)都没通过顺序类似搜索方法被识别。一条随机的森林途径在proteome识别了388个通常认为的法新社。法新社基因ep-afp在Escherichiacoli被表示，并且表示蛋白质展出了0.97°C的一项热磁滞现象活动，建议它处于E的深supercooling能力的潜在的角色。pela。

简介：Theexpressedproteinswereextractedfromhumanbenignprostatichyperplastictissuesobtainedwithtransurethralresectionoftheprostatebeforeandaftertheirirradiationwithradioactivenuclide.Theproteinswereseparatedbytwo-dimensionalgelelectrophoresisandanalyzedbymassspectrometry.Fourproteinsweredifferentiallyexpressedandwereidentifiedwithadatabasesearch.ThreewereassociatedwiththeregulationofcellmotionandonewaslactatedehydrogenaseB,whichplaysanimportantroleintheprocessofcellenergymetabolismandcellstatechangesintissue.Thecomparativeproteomicanalysisindicatesthatafterirradiationwithradioactivenuclide,changesoccurinhumanbenignprostatichyperplasticcells,andassociatedproteinsareexpressed.Analysisoftheseproteinsshouldhelptoidentifythemechanisminvolvedwhenhumanprostatichyperplasiaistreatedbyirradiationwithradioactivenuclide.

简介：BACKGROUND:Theexpressionofubiquitinandenergy-associatedproteincanprovokemigraines.Studieshavesuggestedthatexpressioniscloselylinkedto'hyperactivityofliver-yangtheory'inTraditionalChineseMedicine(TCM),aswellasthefunctionofperipherysympatheticnervemedulla.OBJECTIVE:Toobserveproteomicchangesinaratmigrainemodelwithregardtohyperactivityofliver-yangwhentreatedwithChineseherbstocalmtheliverandsuppresshyperactiveyangcompound.DESIGN,TIMEANDSETTING:Arandomizedcontrolledstudy.ThisstudywasperformedatthelaboratoryofInstituteofIntegratedTraditionalChineseandWesternMedicine,InstituteofHumanReproductionandStemCellEngineeringandKeyLaboratoryofCancerProteomicsofMinistryofHealth,XiangyaHospitalAffiliatedtoCentralSouthUniversitybetweenSeptember2006andJuly2007.MATERIALS:Thirty,male,healthy,Sprague-Dawleyrats,agedeightweeks,wereincludedinthefinalanalysis.Aconite,tocalmtheliverandsuppresshyperactiveyangcompound,wasprovidedbytheDispensaryofTraditionalChinesemedicine,XiangyaHospital,CentralSouthUniversity.Aphysiologicalelectronicstimulator,typeSDQ-1,wasprovidedbyBengbuPracticalInstituteofTechnology.Thelefttrigeminalganglionwaslocalizedandstimulatedfor10minutes,andtheratswereorallyadministeredanaconiteconcoctiontoestablisharatmigrainemodelwithhyperactivityofliver-yang.METHODS:Ratswererandomlydividedintoanormalcontrolgroup,modelgroup,andTCMtreatmentgroup,with10ratsineachgroup.TheTCMtreatmentgroupwasorallytreatedtocalmtheliverandsuppressthehyperactiveyangcompoundonceadayfor28days.Incontrast,themodelgroupandnormalgroupwereorallyadministeredthesameamountofdistilledwateronceadayfor28days.MAINOUTCOMEMEASURES:Thetotalproteinsfromadrenalglandsofthethreegroupswereseparatedbytwo-dimensionalgelelectrophoresis(2-DE),and2-DEimageswereanalyzedbyPDQuest7.0

简介：巨噬细胞有效地通过杀死肿瘤房间的一主要机制要求房间到房间接触，显示在激活的巨噬细胞的房间表面上表示的某些分子可以调停tumoricidal能力。肿瘤坏死因素(TNF)和氮的氧化物(没有)是肿瘤房间death.However的二个古典调停人，差异的证据正在积累显示这些已知的调停人不看起来说明女人和巨噬细胞的有势力tumoricidal活动。获得tumoricidalactivationassociated膜蛋白质的一个完整的全部剧目，我们把一个维的SDS页与毛状的液体chromatographytandem团spectrometry(LC-MS/MS)相结合。用这种技术，我们鉴别454激活的巨噬细胞明确地与极其高的信心表示了蛋白质，包括巨噬细胞的大多数已知的激活标记，例如没有synthase(iNOS)，Ym1，cyclooxygenase，等等。膜界限TNF-也在激活的巨噬细胞上被识别。然而，它也在thioglycolate上被检测得到的巨噬细胞，显示这个分子不能在变化形式依赖者肿瘤房间杀死起一个关键作用。相反，尽管不没作为变化形式依赖者tumoricidal小径的一个受动器分子被分配，iNOS从激活的巨噬细胞的膜部分被识别，不建议可以涉及变化形式依赖者tumoricidal机制，因为有血浆膜的iNOS协会理想地被适合直接不交付进联系肿瘤房间。这研究提供不仅新卓见进巨噬细胞变化形式依赖者tumoricidal机制，而且巨噬细胞激活的一个珍贵数据集合联系的膜蛋白质，因此在反肿瘤和另外的生物进程提供巨噬细胞的功能的机制的更好的理解。

简介：Differentialproteomeprofilesofhumanlungsquamouscarcinomatissuecomparedtopairedtumor-adjacentnormalbronchialepithelialtissuewereestablishedandanalyzedbymeansofimmobilizedpHgradient-basedtwo-dimensionalpolyacrylamidegelelectrophoresis(2-DPAGE)andmatrix-assistedlaserdesorption/ionizationtimeofflightmassspectrometry(MALDI-TOF-MS).Theresultsshowedthatwell-resolved,reproducible2-DEpatternsofhumanlungsquamouscarcinomaandadjacentnormalbronchialepithelialtissueswereobtainedundertheconditionof0.75-ugprotein-load.Theaveragedeviationofspotpositionwas0.733+0.101mminIEFdirection,and0.925+0.207mminSDS-PAGEdirection.Fortumortissue,atotalof1241±88spotsweredetected,987±65spotswerematchedwithanaveragematchingrateof79.5%.Forcontrol,atotalof1190+72spotsweredetected,and875±48spotswerematchedwithanaveragematchingrateof73.5%.Atotalof864±34spotswerematchedbetweentumorsandcontrols.Forty-threedifferentialproteinswerecharacterized:someproteinswererelatedtooncogenes,andothersinvolvedintheregulationofcellcycleandsignaltransduction.Itissuggestedthatthedifferentialproteomicapproachisvaluableformassidentificationofdifferentiallyexpressedproteinsinvolvedinlungcarcinogenesis.Thesedatawillbeusedtoestablishhumanlungcancerproteomedatabasetofurtherstudyhumanlungsquamouscarcinoma.

简介：AbstractBackground:The human brain is the most complex organ in the body, and it is important to have a better understanding of how the protein composition in the brain regions contributes to the pathogenesis of associated neurological disorders.Methods:In this study, a comparative analysis of the frontal and temporal cortex proteomes was conducted by isobaric tags of relative and absolute quantification (iTRAQ) labeling and two-dimensional liquid chromatography-tandem mass spectrometry (2D LC-MS/MS). Brain protein was taken from relatively normal tissue that could not be avoided of damage during emergent surgery of the TBI (traumatic brain injury) patients admitted in Beijing Tiantan Hospital from 2014 to 2017. Eight cases were included. Four frontal lobes and 4 temporal lobes proteome were analyzed and the proteins were quantitated. Gene Ontology (GO), Ingenuity Pathway Analysis (IPA), and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were used to analyze the biological function of identified proteins, unchanged proteins, and differentially expressed proteins (DEPs).Results:A total number of 2127 protein groups were identified in the frontal and temporal lobe proteomes. A total of 1709 proteins could be quantitated in both the frontal and temporal cortex. Among 90 DEPs, 14 proteins were screened highly expressed in the temporal cortex, including MAPT, SNCG, ATP5IF1, GAP43, HSPE1, STMN1, NDUFS6, LDHB, SNCB, NDUFA7, MRPS36, EPDR1, CISD1, and RALA. In addition, compared to proteins expressed in the frontal cortex, 14 proteins including EDC4, NIT2, VWF, ASTN1, TGM2, SSB, CLU, HBA1, STOM, CRP, LRG1, SAA2, S100A4, and VTN were a low expression in the temporal cortex. The biological process enrichment showed that unchanged proteins between the frontal and temporal cortex mainly take part in regulated exocytosis, axon guidance, and vesicle-mediated transport. The KEGG pathway analysis showed that unchanged proteins between the frontal and temporal cortex mainly take part in oxidative phosphorylation, carbon metabolism, Huntington's disease, and Parkinson's disease.Conclusions:The majority of proteins are unchanged between the frontal and temporal cortex, and unchanged proteins are closely related to its function. Among DEPs, MATP (tau) is upregulated in the temporal cortex, closely related to Alzheimer's disease (AD), and is one of the targets for the treatment of AD. CLU is downregulated in the temporal cortex which functions as an extracellular chaperone that prevents aggregation of non-native proteins. It was suggested that the temporal lobe may not be the "functional dumb area" of the traditional view, but could be involved in important neural metabolic circuits.

简介：