简介：Viaseveraltechniquestomodifysensitivematrixforinhabitingtheleakageoffluorescentindicator,anewstablesensingmaterialformonitoringphosphateshasbeenpreparedandappliedtothemeasurementofphosphatesinartificialseawater.ItisbasedonthereactionofPO3-4withAl(Ⅲ)-Morinthatleadstothefluorescencequenchingofthecompositematrix.AtpHvalue4.0andthesalinityvalue25.0,theresponsetimeofoutputsignalhavingreachedthesteadystateislessthan300seconds.ItscalibrationgraphisgainedintherangeofH2PO-4massconcentrationfrom1.50to7.00μg/mLwiththelimitofdetection(3σ/K)0.02μg/mL.Whenitwasusedtomeasurephosphatesinartificialseawater,therecoveryrangedfrom96.78%to102.09%.Beingstoredunderthepropercondition,themembranesmaintainsensitivefor90-120days,andareabletobeusedfor50-80timeswithindicatorsupplement.

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简介：Theobjectiveofthestudywastoelucidateopticalcharacteristicsofthechromophorestructuresoffluorescentproteins.RamanspectraofcommonlyusedGFP-likefluorescentproteins(FPs)withdiverseemissionwavelengths(green,yellow,cyanandred),includingtheenhancedhomogenousFPsEGFP,EYFP,andECFP(fromjellyfish)aswellasmNeptune(fromseaanemone)weremeasured.High-qualityRamanspectrawereobtainedandmanymarkerbandsforthechromophoreoftheFPswereidentifiedviaassignmentofRamanspectrabands.WereportthepresenceofapositivelinearcorrelationbetweentheRamanbandshiftofCs=C6andtheexcitationenergyofFPs,demonstratedbyplottingabsorptionmaxima(cm-1)againstthepositionoftheRamanbandCs=C6inEGFP,ECFP,EYFP,theanionicchromophoreandtheneutralchromophore.ThisstudyrevealednewRamanfeaturesinthechromophoresoftheobservedFPs,andmaycontributetoadeeperunderstandingoftheopticalpropertiesofFPs.

简介：在这个工作，新奇bisbenzimidazolylpyridine-functionalized荧光灯环氧基树脂树脂为organophosphate杀虫剂被综合察觉。环氧基树脂树脂被Fourier变换红外线的光谱学(英尺红外)描绘，质子原子磁性的回声光谱学(1HNMR)，胶化浸透层析(GPC)，微分扫描热量测定(DSC)和荧光光谱学。在与Eu(III)离子装载以后，环氧基树脂树脂显示出强壮的荧光排出物。当暴露了在答案跟踪diethylchlorophosphate的数量时，荧光排放被观察即刻地被熄灭。熄灭常数K为在617 熄灭的sv；nm决心是0.377 ；× ；103 L/mol。这敏感熄灭排放的功能和聚合支持的容易的处理自然使树脂能是organophosphates的察觉的一个有希望的chemosensor候选人。

简介：Afeasiblemethodofcombiningtheconceptoffluorescencehalf-lifeandthepowerdependentphoto-bleachingrateforcharacterizingthepracticalphotostabilityoffluorescentproteins(FPs)wasintroduced.Furthermore,byusingafluorescentphotostabilitystandard,arelativecomparisonofthephotostabiltyofFPsfromdifferentresearchgroupswasproposed,whichwouldbeofgreatbenefitfordevelopingnovelFPswithoptimizedemissionwavelength,betterbrightness,andimprovedphotostability.Weusedrho-damineBasanexampletoverifythismethodandevaluatethepracticalphotostabilityofafar-redFP,mKate-S158C.Experimentalresultsindicatedgoodpotentialofthismethodforfurtherstudy.

简介：Anegativeunipolarpulsedvoltageisappliedtostudyinternalelectricalparametersofthexenonfilleddielectricbarrierdischarge（DBD）sources.TheVUVradiationemittedfromthesesourcesischaracterizedbymeansofthephotoluminescenceintensityoftheredphosphorpellet.TheredphosphorconvertstheVUVradiationintovisibleradiationandtheemissionspectraincludeapeakat619.56nm.Theemissioncharacteristicsoftheredphosphorareanalyzedintermsofthepressure-distance（pd）,risetimeandfrequencyofthepulsedvoltagewaveform.Theemissionintensitymeasuredatdifferentoperationalconditionsconfirmsthattheformationanddecayofthexenonexcimer,Xe2*,increasewiththeincreaseinreducedelectricfield,E/N.AfterexceedingcertainlimitsofE/N,theintensityofXe2*decreasesrapidly.

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简介：Thebiogenicmonoaminesdopamine(DA),norepinephrine(NE)andserotonin(5-hydroxytryptamine,5-HT)aremajorneuromodulatorsinthemammaliancentralnervoussystem(CNS).DAcontainingneuronsarefoundini)themesolimbicsysteminwhichcellbodiesintheventraltegmentalarea(VTA)projectaxonsintotheamygdala,cortex,hippocampusandthe

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简介：Parameterquantificationprincipleofthree-dimensional(3-D)fluorescentspectrumanditsrealizationtechniquearestudied.Thepotablefiberopticfluorescentmeasurerformineraloilisgiven.Threeparametersoftheexcitinglightwavelength.theeavelengthandtheintensityofthefluorescentlightexcitedbythemeasuredoilareexpressedwith3-Dcoordinatesandareprocessedsoastobesampledagainandtobecurvefitted.

简介：Primordialgermcells(PGCs),asprecursorsofmammaliangermlineage,havebeengainingmoreattentionasanewresourceofpluripotentstemcells,whichbringagreatpossibilitytostudydevelopmentaleventsofgermcellinvitroandatanimallevel.EG4cellsderivedfrom10.5dayspostcoitum(dpc)PGCsof129/svJstrainmousewereestablishedandmaintainedinanundifferentiatedstate.WithanattempttostudythedifferentiationcapabilityofEG4cellswithareporterprotein:greenfluorescenceprotein,andthepossibleapplicationofEG4cellsintheresearchofgermcelldevelopment,wehavegeneratedseveralEG4-GFPcelllinesexpressingenhancedgreenfluorescenceprotein(EGFP)andstillmaintainingtypicalcharacteristicsofpluripotentstemcells.Then,thedifferentiationofEG4-GFPcellsinvitroaswellastheirdevelopmentalfateinchimericembryoswhichwereproducedbyaggregatingEG4-GFPcellsto8-cellstageembryoswerestudied.TheresultsshowedthatEG4cellscarryinggreenfluorescencehaveapotentialuseintheresearchofgermcelldevelopmentandotherrelatedstudies.

简介：Wereportonanoveldibenzothiophene-basedtwo-photonfluorescentprobeforselectivenuclearbioimaging,whichcontainsbilaterallysymmetricalpyridineringsconnectedbyacentralconjugated-bridgedibenzothiophene.Thisprobepossessesalargetwo-photonabsorptioncross-sectionof471GM,yieldsa25-foldenhancementofthefluorescencetitration,andastrongerphotostabilityfornucleilabelingthanexistingprobes.Thereal-timeobservationperiodisaminimumof1800sunderafemtosecondlaserexcitation,whichissignificantlylongerthanthatof40,6-diamidino-2-phenylindole.Theaboveresultsconfirmthatthisnovelmoleculeisasuitabletwo-photonfluorescentprobeforapplicationtonuclearbioimagingincells.

简介：AgreatamountoffoodbornepathogenswereGram-positive(G?)bacteria,athreattopublichealth.Inthisstudy,consideringthebindingabilityofnisintowardsG-bacteriaandthestablefluorescentabilityofEGFPprotein,afluorescentnisin–EGFPproteinprobewasconstructedbyageneengineeringmethod.NisinandEGFPwereusedasthereceptorandfluorophore,respectively,todetectG?bacteria.ThenisinandegfpgenewereamplifiedseparatelyaccordingtothesequencepublishedinGenBankusinguniqueprimers.ThetwogeneswereclonedintoapET-28b(?)vectorresultinginapET-28b(?)–nisin–egfpvector.ThevectorwastransferredintoEscherichiacoli(E.coli)BL21(DE3)forexpression.Theexpressedproteinwasextracted,purifiedbyaNi–NTAcolumn,andthentestedbytheSDS-PAGEmethodtoconfirmitsmolecularweight.Listeriamonocytogenes(L.monocytogenes),Staphylococcusaureus(S.aureus),andMicrococcusluteus(M.luteus)wereusedastherepresentationsofG?bacteria.E.coliO157,representingthegram-negative(G-)bacteria,wasusedasanegativecontrol.Thebindingspecificityoftherecombinantproteinwasperformedontwotypesofbacteriaandthendetectedthroughfluorescentmicroscopy.Theresultsindicatedthatthenisin–EGFPprobecoulddetectG?bacteriaat108CFU/mL.

简介：Genomicresearchhasmadealargenumberofsequencesofnovelgenesorexpressedsequencetagsavailable.Toinvestigatefunctionsofthesegenes,asystemforconditionalcontrolofgeneexpressionwouldbeausefultool.Inducibletransgeneexpressionthatusesgreenfluorescentproteingene(gfp)asareportergenehasbeeninvestigatedintransgeniccelllinesofcotton(COT;GossypiumhirsutumL.),Fraserfir[FRA;Abiesfraseri(Pursh)Poir],Nordmannfir(NOR;AbiesnordmannianaLk.),andrice(RIC;OryzasativaL.Cv.Radon).Transgeniccelllineswereusedtotestthefunctionofthechemicalinducerdexamethasone.Inducibletransgeneexpressionwasobservedwithfluorescenceandconfocalmicroscopy,andwasconfirmedbynorthernblotanalyses.Dexamethasoneat5mg/Linducedgfpexpressiontothenearlyhighestlevel48haftertreatmentinCOT,FRA,NOR,andRIC.Dexamethasoneat10mg/LinhibitedthegrowthoftransgeniccellsinFRAandNOR,butnotCOTandRIC.Theseresultsdemonstratedthatconcentrationsofinducerforoptimuminduciblegeneexpressionsystemvariedamongtransgeniccelllines.Theinduciblegeneexpressionsystemdescribedherewasveryeffectiveandcouldbevaluableinevaluatingthefunctionofnovelgene.

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简介：ZnOnanotubeswerehydrothermallysynthesizedandtheZNTs/CeO2fluorescentcompositewerepreparedbyintroducingnanoCeO2particlesintoZnOnano-tubesviaachemicalsolutionadsorptionandannealingprocess.ThesampleswerecharacterizedbyX-raydiffraction,scanningelectronmicroscopy,Fouriertransformationinfraredspectroscopyandroomtemperaturephotoluminescencemeasurement.DuetotheinteractionbetweenCe4+andthesurfaceatomsofZnO2nano-tubes,aphotoluminescenceenhancementwasobser...

简介：Novelmolecularmaterial,1-benzothiazoly-3-pheny1-pyrazoline(BTPP)wasfoundtofunctionasbrightbluelightemittingdyeinorganicelectroluminescentdevice,anditsopticalandelectriccharacteristicswereinvestigated.Thisheterovycliccompoundexhibitedgoodcharacteristicsofbluephotoluminescenceandelectroluminescence,whichhadtheemissionpeakat450nm.Thesinglelayerlight-emittingdevicesusingBTPPaslight-emittingmaterialdispersedinpoly(N-vinylcarbazole)(PVK)anddoublelayeronesusingPBDasholeblocklayerabovethelight-emittinglayerwerefabricatedusingconventionalspin-castingandvaccumvapourdepositionmethods.TheintroductionofPBDhasenhancedelectroninjectionandluminanceefficiency,comparedwiththesinglelayerLEDs.

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简介：扩大来源的阶段信息与一个环形的二地区的阶段唯一的过滤器被重建在一荧光灯的共焦的扫描为分辨率的光显微镜改进。它来源尺寸上并且在过滤器的外部环形的地区的阶段传播上的决定的依赖被它的三维的光转移功能(3DOTF)的使用理论上调查。增加的来源尺寸和为光系统的最佳的3DOTF的阶段唯一的过滤器的外部环形的地区的要求的阶段价值被介绍。CLC数字TH742