简介:AsapartofabasicresearchprojectonXeno-transplantion,wehavebeenengagedinthederivationofembryonicstemcelllinesfromChineseminiswine.Here,wereportedforthefirsttimetheestablishmentoftwoporcineEGcelllines(BPEG1andBPEG2)fromprimordialgermcellsofgenitalridgesofa28anda27dembryosrespectively.Theirpluripotentnaturehasbeenidentifiedbycolonymorphology,markercharacterizationaswellasbyinvitroandinvivodifferentiation.TheseporcineEGcellsarepotentiallyusefulforfurtherbasicstudies.