简介：Objective:Thispaperaimsatmeasurementenhancedeffectofoxidizedlipoprotien(a)[oxLp(a)]onpermeabilityofmonolayerendothelialcellsandrelationshipwithreactiveoxygenspecies(ROS)generationanddesmogleins(DSGs)expression.MethodsandResults:Transendothelialpermeabilitywasassayedbytranswellandreactiveoxygenspecies(ROS)wasdeterminedbyDCFH-DAstaining.RT-PCRwascarriedouttodetermineDSGlandDSC2expressioninmRNA,respectively.TransendothelialpermeabilitywasenhancedbyoxLP(a)doseandtimedependently.Themostmarkedeffectappearedataconcentrationof100mg/L,Transendothelialpermeabilityreachedthemaximumvalueafter2hofFITC-dextranaddition,andthengraduallydecreasedafter4h.oxLp(a)inducesthegenerationofcellularreactiveoxygenspecies(ROS),andthiseffectcouldbeinhibitedbysuperoxidedismutase(SOD).IncubationofHUVECswithoxLp(a)resultedinadoseandtime-dependentdown-regulationofDSGlandDSC2expressionattranscriptionallevel.Conclusion:PermeabilityofmonolayerendothelialcellswasenhancedbyoxLp(a)whichisrelatedtoup-regulatingROSformationanddown-regulatingdesmogleinsexpression.