简介：Thesusceptibilityoftheclavicletoinjuryunderscoresitsroleasanimportantloadingpathduringbothfrontalimpactandsideimpactcrashes,sinceclavicleisloadeddirectlybyshoulderbeltinfrontalimpactandthroughshoulderinlateralimpactcrashes.Thisstudyaimstodevelopingaparametricclaviclefiniteelementmodelcapableofsimulatingimpactresponseswithdifferentparameters.Astatisticalclaviclegeometrymodelwasdevelopedbasedoncomputedtomography(CT)scansfrom89subjectsusingprincipalcomponentanalysis(PCA)andmultivariateregressionanalysis.Usingthisstatisticalclaviclegeometrymodelandmeshmorphingmethoddevelopedinthisstudy,aseriesofdetailed3Dclaviclegeometrieswerepredictedbyage,gender,andlengthofthebone,allofwhichshowedstrongeffectsonclaviclegeometry.Afterassigningmaterialpropertiesandvalidationprocess,thisparametricmodeldevelopedinthisstudycanbeusedtoinvestigatetheeffectsofage,genderandlengthatthesametime.

简介：Objective:Thispaperaimsatmeasurementenhancedeffectofoxidizedlipoprotien(a)[oxLp(a)]onpermeabilityofmonolayerendothelialcellsandrelationshipwithreactiveoxygenspecies(ROS)generationanddesmogleins(DSGs)expression.MethodsandResults:Transendothelialpermeabilitywasassayedbytranswellandreactiveoxygenspecies(ROS)wasdeterminedbyDCFH-DAstaining.RT-PCRwascarriedouttodetermineDSGlandDSC2expressioninmRNA,respectively.TransendothelialpermeabilitywasenhancedbyoxLP(a)doseandtimedependently.Themostmarkedeffectappearedataconcentrationof100mg/L,Transendothelialpermeabilityreachedthemaximumvalueafter2hofFITC-dextranaddition,andthengraduallydecreasedafter4h.oxLp(a)inducesthegenerationofcellularreactiveoxygenspecies(ROS),andthiseffectcouldbeinhibitedbysuperoxidedismutase(SOD).IncubationofHUVECswithoxLp(a)resultedinadoseandtime-dependentdown-regulationofDSGlandDSC2expressionattranscriptionallevel.Conclusion:PermeabilityofmonolayerendothelialcellswasenhancedbyoxLp(a)whichisrelatedtoup-regulatingROSformationanddown-regulatingdesmogleinsexpression.

简介：Segmentingwholeheartfromcardiaccomputedtomography(CTimagescanprovideanimportantbasisfortheevaluationofcardiacfunctionandhelpimprovetheaccuracyofclinicaldiagnosis.Manualsegmentationisthemostaccuratemethodforcardiacsegmentation.Butitistimeconsumingandnotsufficientlyreproducible.However,cliniciansstillrelyonthismethodinpracticalapplications.Soafullyautomaticmethodisneededtoimprovethesegmentationefficiency.Thispapeproposesaregistration-basedautomaticapproachforthree-dimensional(3DsegmentationofcardiacCTimages.TheproposedmethodutilizesthesimilarityocardiacCTimagesbetweendifferentindividuals,andusesregistrationtoachievethesegmentation.Affinetransformationisfirstlyimplementedtoachieveglobalcoarseregistration.Then,cubicB-splinesareusedtorefinethelocaldetailsinlocallyaccurateregistration.Mutualinformation(Ml)isusedasthesimilaritymeasure,andadaptivestochasticgradientdescent(ASGD)astheoptimizationalgorithm.Oumethodisappliedtothedual-sourcecardiacCTimagestosegmentwholeheartExperimentalresultsshowthattheproposedmethodcanautomaticallysegmentwholeheartfromcardiacCTimages.

简介：Propagation-basedphase-contrastimagingwassimulatedbasedonparaxialFresnel-Kirchoffdiffractionintegralandsphericalwaveillumination.Underadevelopedmicro-CTsystemparameters,theeffectsoffocal-spotsizeandimaginggeometryonphase-contrastimaginghavebeeninvestigatedusinga2-mm-thicknesspolystyreneedgephantom.Anequivalentmono-energywasusedtosubstitutethepolychromaticspectrumofthemicro-focusX-raysource.Toconsidereffectsoffocal-spotsizeanddetectorresolution,theobtainedphase-contrastimagewithanidealpointsourcewasconvolvedwithsourceintensitydistributionandpointspreadfunctionofdetector.Simulationsshowreasonableinfluencesofthetwoparameterswhichareingoodagreementwithexperimentalresults.

简介：Interfacedebondingbetweenprosthesesandabutmentswasthemostfrequentfailuremodeofresin-bondedfixedpartialsdentures(RBFPDs)inclinic.ThepurposeofthisstudywastoinvestigatetheeffectofaccessoryretentionformsonthebondstrengthofRBFPDs.Threetypesof3Dfiniteelementmodelswereconstructed.ThemodelofposteriormetalplateRBFPDwithspoon-shapedocclusalrestseatsservedasthecontrol.Theremainingtwotypesofmodelsbasedonthecontroladdedtheretentionformdesignofthepinholeandaxialgrooverespectively.Theaxialorbuccolingualloadof150Nwasappliedontheprosthesis,firstpremolarandfirstmolarrespectively.Themaximumprincipalstressesoftheadhesivelayerindifferentmodelswerecalculated.Undertheloadofthesamemagnitude,thestressduetothebuccolingualloadwassignificantlyhigherthanthatduetotheaxialloadintheadhesivelayer.Theproximalshoulder,occlusalrestseatswallandtheproximalmarginadjacenttotheshoulderwerethehighriskregionwheretheadhesivelayerdamagedeasily.Comparedwiththecontrolmodel,thepinandgroovemodelscouldslightlydecreasethestressintheadhesivelayeraftertheaxialloading,whilethestressintheadhesivelayerdrasticallydecreasedafterhorizontalloadingandreducedby22%and31%respectively.TheseresultsindicatethatthehorizontalocclusalforcehasamoreseriousharmtothedebondingofRBFPDs.Inaddition,theaccessoryretentionforms(e.g.pinandaxialgrooveretentionforms)candecreasethestresslevelintheadhesivelayer,whichareconducivetoincreasetheload-bearingcapacityofRBFPDs.

简介：Inthispaper,weproposeamethodtoimproveanti-radiationcapabilitybycoatingheavymetalX-rayprotectionglasswithcompoundphotoniccrystallayers,basedontheuniquepropertyofphotoniccrystalthatlightcannotbepropagatedwithintherangeofbandgaps.Usingtheplanewaveexpansionmethod,wemadeatheoreticalstudyofparametersaffectingthebandgapstructuresofone-dimensionalphotoniccrystals.Basedonthefindings,wechoseappropriatematerialsandcompoundstructureofphotoniccrystalsoastogethighX-rayreflectioncoatingphotoniccrystallayers.Bythismethod,thereflectionratewithinX-raywavelengthcanreachthemaximumvalueof100%,andtheaveragevalueofover90%.Evenlow-costheavymetalX-rayprotectionglassofabsorptioncoefficientvaluecanachievethedesiredeffect.Thus,thismethodgreatlydecreasestheanti-radiationrequirementsofheavyX-rayprotectionglass.

简介：Thecompressedsensing(CS)ofaccelerationdatahasbeendrawingincreasingattentioningaittelemonitoringapplication.Insuchapplication,therestillexistsomechallengingissuesincludinghighenergyconsumptionofbody-worndeviceforaccelerationdataacquisitionandthepoorreconstructionperformanceduetononsparsityofaccelerationdata.Thus,thenovelschemeofcompressivesensingofaccelerationdataisneededurgentlyforsolutionsthatarefoundtotheseissues.

简介：Phasecontrastimagingtechniquehasbeenimprovedpromptlyinrecentyears.Amongthesetechniquesin-linephase-contrastimagingiswidelyused.Variousalgorithmsforin-linephaseretrievalhavebeenproposedsofarsuchasTIE(transportofintensityequation),CTF(contrasttransferfunction),firstborn-approximations,GSF(Gerchberg-Saxton-Fienup),etc.Bronnikov'salgorithm(BA)isatypeoflinearalgorithmthatissimpleandefficient.Butitcanonlybeusedwithoutabsorptionsituations.Inthispaper,animprovedalgorithmbasedonBAwaspresented.TheapproachaddsΔφ(x,y)tothephasemapφ_b(x,y)retrievedbyBAtomakethereconstructedphasemapmoreprecise.Further,theapproachwasevaluatedonsimulatedimagesandconfirmedtobeaccurateathigherabsorptionrates.

简介：Alongwiththeincreasinglyurgentmanagementneedsofintranetterminalsinhospital,andlargescaleddeploymentofterminalmanagementsystem,terminalaccesscontrolhasbecomeoneofthestandardfunctionsofterminalmanagement.Thispapermainlyaimsatsomesimpleresearchforthesystemconstructionofhospitalintranetterminalaccesscontrol.

简介：Usingofactivecardiacmedicaldevicesincreasessteadily.InEurope,therewere183implantsofICDand944implantsofPM,119ofbiventricularICDand41ofbiventricularPM,allpermillionin-habitantsin2014.Healthcareenvironments,includingradiotherapytreatmentrooms,areconsideredchallengingfortheseimplantabledevices.Exposuretoradiationmaycausethedevicetoexperienceprematureelectivereplacementindicator,decreasedpacingamplitudeorpacinginhibition,inappropri-ateshocksorinhibitionoftachyarrhythmiatherapyandlossofdevicefunction.

简介：Theergonomicdigitaldesignandevaluationofman-machinesystemisthemainapplicationfieldsofvirtualhumantechnology.Becauseofthecomplexityofphysicalpersonanduncertaintyoffatiguegenerationmechanism,itisdifficulttoexpressandevaluatetheVirtualHuman'sfatiguestate.Thispapersummarizesthecharacteristicsofvirtualhuman'sfatigueevaluation,suchasundeterminable,invisible,theconstraintconditionscanbeenused.Viewedfromthestrengthsandweaknessesofpreexistingmodels,metabolicenergyexpendituremodel,dynamicmusclefatiguemodelandamodeltoassessfatigueatjoint-levelusingthehalf-jointconcept,thispaperputsforwardthefuturedevelopmenttrendthatthecombinationofsubjectiveandobjective,diversificationoftheanalyzablemotion,real-timeandvisualexpression.

简介：LargeconductanceCa~(2+)-activatedK~+(BK_(Ca))channelexhibitsaphenotype-dependentexpressiononvascularsmoothmusclecells(VSMCs),whichpreferstocontractilephenotype.Meanwhile,shearstressdefinitelyinfluencesVSMCsproliferationandcontraction.Thereby,ahypothesiswasraised,wouldshearstresschangetheBK_(Ca)expressionandcorrelatewithVSMCphenotype?Inordertoinvestigateit,VSMCswereexposedtoshearstressinaparallel-plateflowchamberwith12dynes/cm~2for12h.Subsequently,theeffectofshearstressonVSMCproliferation,BK_(Ca)channelexpressionandcontractilephenotypemarker,α-smoothmusclecellactin(α-SMA)andsmoothmusclemyosinheavychain(SMMHC),wasdeterminedbyimmunofluorescencemicroscopy,flowcytometeryaswellasreversetranscriptionpolymerasechainreaction(RT-PCR),respectively.DatashowthatshearstressenhancedtheexpressionofBK_(Ca)channelwhileinhibitingVSMCproliferation.Paralleledtothosephenomena,theexpressionofbothα-SMAandSM-MHCweredecreasedsignificantly.TheseresultsdemonstratedthatupregulationofBK_(Ca)channelwasirrelevanttothemaintenanceVSMCofcontractilephenotypeundershearstress.ThisfindingprovidesanewinsightintounderstandingthecorrelationofBK_(Ca)channelandVSMCphenotype.

简介：Areaswithhighfrequencyactivitywithintheatriumarethoughttobe'drivers'oftherhythminpatientswithatrialfibrillation(AF)andablationoftheseareasseemstobeaneffectivetherapyine-liminatingDFgradientandrestoringsinusrhythm.ClinicalgroupshaveappliedthetraditionalFFT-basedapproachtogeneratethethree-dimensionaldominantfrequency(3DDF)mapsduringelectro-physiology(EP)proceduresbutliteratureisrestrictedonusingalternativespectralestimationtech-niquesthatcanhaveabetterfrequencyresolutionthatFFT-basedspectralestimation.

简介：目的寻找一种简便、有效的方法,从股静脉同时获取内皮细胞和肌成纤维细胞.方法取犬股静脉5cm,将静脉内膜外翻,两端内套、夹闭,胶原酶消化法获取内皮细胞,去内皮的静脉采用组织块贴壁法获取肌成纤维细胞.结果应用本方法获得的内皮细胞纯度达99.19%,7～9天即可获得1×106个肌成纤维细胞,而细胞的增殖分化能力并未受影响.结论本方法简便、易行,获得的内皮细胞纯度高,肌成纤维细胞培养周期缩短.

简介：

简介：目的了解人工肝支持系统(ALSS)治疗重症肝炎临床疗效.方法应用WLXGX-888型人工肝支持系统(北京伟力公司生产)对24例重症肝炎进行血浆置换、血液滤过等方法治疗40例次.对患者治疗前后的肝功能、前白蛋白(PA)、凝血酶原活动度(PTa)、血氨、电介质进行分析.结果ALSS治疗后患者的肝功能明显改善,总胆红素(TBIL)、血氨均明显下降(P<0.001),总蛋白下降(P<0.001);白蛋白下降,但无显著性差异(P>0.05);前白蛋白提高明显(P<0.001).亚急性、慢性重症肝炎的早、中期治愈好转率分别为71.43%、44.44%,而晚期则无一例存活.结论ALSS治疗重症肝炎安全,效果显著,可明显提高重症肝炎的生存率.

简介：目的探讨人工肝血浆置换法治疗重症肝炎的疗效及影响疗效的因素.方法32例重症肝炎患者在内科治疗基础上给予血浆置换治疗52例次,血浆置换量平均3000ml.结果32例重症肝炎患者经血浆置换后,临床症状都得到一定程度的改善,肝功能明显好转.总有效率为62.5%,其中早期为87.5%,中期为70.59%;与对照组相比,总胆红素由治疗前377.1mmol/L降至197.6mmol/L(P<0.01).结论血浆置换对重症肝炎中、早期治疗效果较好.

简介：

简介：目的评估组合生物型人工肝治疗慢性重症乙型肝炎的临床疗效.方法将KM8800型血液净化仪与BIOLIVA3A型生物型人工肝机连接成组合生物型人工肝装置,对4例晚期慢性重症乙型肝炎患者共实施5例次治疗.先进行患者血液血浆置换,再经过生物型人工肝处理.观察患者治疗前、后的临床表现;比较肝功能各相关指标的变化,并比较血浆置换后、生物型人工肝处理后回路血中相应指标的变化.结果本组患者治疗过程中生命体征平稳,除1例次出现过敏反应外,未发生其它不良反应.与治疗前比较,治疗后即刻患者血中总胆红素(TBIL)、丙氨酸转移酶(ALT)、氨(NH3)明显减少(P<0.01),高密度脂蛋白(HDL)、纤维蛋白原(FIB)明显增加(P<0.01),治疗后第3天凝血酶原活动度(PTa)明显提高(P<0.01);与血浆置换后比较,生物型人工肝处理后回路血中FIB有所增加(P<0.05),而NH3明显降低(P<0.01).结论应用组合生物型人工肝治疗慢性重症乙型肝炎是安全、有效的.组合生物型人工肝比单纯血浆置换疗效好.

简介：目的探讨经乙状窦后径路行前庭神经切断术(vestibularneurotomy，VNT)控制梅尼埃病眩晕症状中，辅助应用内镜微创技术的方法及其减轻术中脑组织压迫和减少术后并发症发生率的价值和意义。方法临床观察乙状窦后径路常规VNT(12例)与辅助应用内镜技术VNT(9例)的2组手术患者手术前后症状控制效果、面神经功能及其听觉和前庭生理功能变化结果。结果常规VNT组患者术后有2例发生脑水肿，需行脱水、降压等处理；平均咱阈上升>15dB者2例；平衡功能代偿所需时间平均为(29．00±9．60)天。辅助应用内镜的VNT组术后平均听阈上升>15dB者2例；平衡功能代偿所需时间平均为(28．56±7．91)天。2组术后均未出现面瘫等并发症；术后2年内再发作眩晕，常规手术组有2例(分别发作1次和4次)，内镜手术组有1例(发作2次)。结论经乙状窦后径路行VNT是目前普遍采用的一种较为方便、安全的控制或消除梅尼埃病患者眩晕症状手术方法，同时能保存听觉功能和面神经完整性；手术中辅助应用内镜技术，使该手术操作成为微创和安全，可有效地减轻对脑组织的压迫和减少术后脑水肿发生，未出任何由此而导致的并发症。